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Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence
Zhang, JB; Huang, H; Cai, ZP; Huang, LM; jbzhang30@sina.com.cn
2006
Source PublicationFOOD CONTROL
ISSN0956-7135
Volume17Issue:7Pages:557-563
AbstractA molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.
DepartmentChinese Acad Sci, S China Sea Inst Oceanog, LED, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
KeywordIdentification Red Snappers Semi-nested Pcr 12s Rrna Gene
Subject AreaFood Science & Technology
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Indexed Bysci
Funding ProjectLED
WOS IDWOS:000235266600009
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/5817
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorjbzhang30@sina.com.cn
Recommended Citation
GB/T 7714
Zhang, JB,Huang, H,Cai, ZP,et al. Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence[J]. FOOD CONTROL,2006,17(7):557-563.
APA Zhang, JB,Huang, H,Cai, ZP,Huang, LM,&jbzhang30@sina.com.cn.(2006).Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence.FOOD CONTROL,17(7),557-563.
MLA Zhang, JB,et al."Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence".FOOD CONTROL 17.7(2006):557-563.
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