Knowledge Management System Of South China Sea Institute of Oceanology,CAS
| Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence | |
| Zhang, JB; Huang, H; Cai, ZP; Huang, LM; jbzhang30@sina.com.cn | |
| 2007 | |
| 发表期刊 | FOOD CONTROL
 |
| ISSN | 0956-7135 |
| 卷号 | 18期号:11页码:1331-1336 |
| 摘要 | A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitocliondrial 12S rRNA gene, which were about 450 bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR amplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, L. erythopterus from L. argentintaculatus, L. malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for differentiation of L. sattguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The seminested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Published by Elsevier Ltd. |
| 部门归属 | Chinese Acad Sci, LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China |
| 关键词 | Identification Red Snappers Semi-nested Pcr 12s Rrna Gene |
| 学科领域 | Food Science & Technology |
| URL | 查看原文 |
| 收录类别 | sci |
| 资助项目 | LED |
| WOS记录号 | WOS:000247570600001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.scsio.ac.cn/handle/344004/5885 |
| 专题 | 热带海洋环境国家重点实验室(LTO) |
| 通讯作者 | jbzhang30@sina.com.cn |
| 推荐引用方式 GB/T 7714 | Zhang, JB,Huang, H,Cai, ZP,et al. Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence[J]. FOOD CONTROL,2007,18(11):1331-1336. |
| APA | Zhang, JB,Huang, H,Cai, ZP,Huang, LM,&jbzhang30@sina.com.cn.(2007).Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence.FOOD CONTROL,18(11),1331-1336. |
| MLA | Zhang, JB,et al."Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence".FOOD CONTROL 18.11(2007):1331-1336. |
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