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学科主题: Food Science & Technology
题名: Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence
作者: Zhang, JB ; Huang, H ; Cai, ZP ; Huang, LM
通讯作者: jbzhang30@sina.com.cn
关键词: identification ; red snappers ; semi-nested PCR ; 12S rRNA gene
刊名: FOOD CONTROL
发表日期: 2007
卷: 18, 期:11, 页:1331-1336
收录类别: sci
部门归属: Chinese Acad Sci, LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
项目归属: LED
摘要: A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitocliondrial 12S rRNA gene, which were about 450 bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR amplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, L. erythopterus from L. argentintaculatus, L. malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for differentiation of L. sattguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The seminested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Published by Elsevier Ltd.
原文出处: 查看原文
WOS记录号: WOS:000247570600001
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5885
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Recommended Citation:
Zhang, JB; Huang, H; Cai, ZP; Huang, LM.Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence,FOOD CONTROL,2007,18(11):1331-1336
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文件名: Species identification in salted products of red snappers by semi-nested PCR–RFLP based on the mitochondrial 12S rRNA gene sequence .pdf
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