Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence
Zhang, JB; Huang, H; Cai, ZP; Huang, LM; jbzhang30@sina.com.cn
2007
发表期刊FOOD CONTROL
ISSN0956-7135
卷号18期号:11页码:1331-1336
摘要A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitocliondrial 12S rRNA gene, which were about 450 bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR amplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, L. erythopterus from L. argentintaculatus, L. malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for differentiation of L. sattguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The seminested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Published by Elsevier Ltd.
部门归属Chinese Acad Sci, LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
关键词Identification Red Snappers Semi-nested Pcr 12s Rrna Gene
学科领域Food Science & Technology
URL查看原文
收录类别sci
资助项目LED
WOS记录号WOS:000247570600001
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5885
专题热带海洋环境国家重点实验室(LTO)
通讯作者jbzhang30@sina.com.cn
推荐引用方式
GB/T 7714
Zhang, JB,Huang, H,Cai, ZP,et al. Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence[J]. FOOD CONTROL,2007,18(11):1331-1336.
APA Zhang, JB,Huang, H,Cai, ZP,Huang, LM,&jbzhang30@sina.com.cn.(2007).Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence.FOOD CONTROL,18(11),1331-1336.
MLA Zhang, JB,et al."Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondria 12S rRNA gene sequence".FOOD CONTROL 18.11(2007):1331-1336.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Species identificati(436KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。