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学科主题: Biotechnology & Applied Microbiology
题名: Secretory expression of a heterologous nattokinase in Lactococcus lactis
作者: Liang, XB ; Zhang, LX ; Zhong, J ; Huan, LD
通讯作者: zhongj@im.ac.cn ; huanld@im.ac.cn
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2007-01-01
卷: 75, 期:1, 页:95-101
收录类别: sci
部门归属: Chinese Acad Sci, Inst Microbiol, Ctr Metab Engn Microorganisms, Beijing 100080, Peoples R China; Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resource, Beijing 100080, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Chinese Acad Sci, Sea Inst Oceanol, Guangzhou 510301, Peoples R China
项目归属: LED
摘要: Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus lactis. Promoter P (nisZ) and signal peptide SPUsp were used for inducible and secretory expression of nattokinase in L. lactis. Western blotting analysis demonstrated that nattokinase was successfully expressed, and about 94% of the enzyme was secreted to the culture. The recombinant nattokinase showed potent fibrinolytic activity, equivalent to 41.7 urokinase units per milliliter culture. Expression and delivery of such a fibrinolytic enzyme in the food-grade vehicle L. lactis would facilitate the widespread application of nattokinase in the control and prevention of thrombosis diseases.
原文出处: 查看原文
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5895
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Recommended Citation:
Liang, XB; Zhang, LX; Zhong, J; Huan, LD.Secretory expression of a heterologous nattokinase in Lactococcus lactis,APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(1):95-101
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