Secretory expression of a heterologous nattokinase in Lactococcus lactis
Liang, XB; Zhang, LX; Zhong, J; Huan, LD; zhongj@im.ac.cn; huanld@im.ac.cn
2007
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号75期号:1页码:95-101
摘要Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus lactis. Promoter P (nisZ) and signal peptide SPUsp were used for inducible and secretory expression of nattokinase in L. lactis. Western blotting analysis demonstrated that nattokinase was successfully expressed, and about 94% of the enzyme was secreted to the culture. The recombinant nattokinase showed potent fibrinolytic activity, equivalent to 41.7 urokinase units per milliliter culture. Expression and delivery of such a fibrinolytic enzyme in the food-grade vehicle L. lactis would facilitate the widespread application of nattokinase in the control and prevention of thrombosis diseases.
部门归属Chinese Acad Sci, Inst Microbiol, Ctr Metab Engn Microorganisms, Beijing 100080, Peoples R China; Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resource, Beijing 100080, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Chinese Acad Sci, Sea Inst Oceanol, Guangzhou 510301, Peoples R China
学科领域Biotechnology & Applied Microbiology
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收录类别sci
资助项目LED
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5895
专题热带海洋环境国家重点实验室(LTO)
通讯作者zhongj@im.ac.cn; huanld@im.ac.cn
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GB/T 7714
Liang, XB,Zhang, LX,Zhong, J,et al. Secretory expression of a heterologous nattokinase in Lactococcus lactis[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(1):95-101.
APA Liang, XB,Zhang, LX,Zhong, J,Huan, LD,zhongj@im.ac.cn,&huanld@im.ac.cn.(2007).Secretory expression of a heterologous nattokinase in Lactococcus lactis.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,75(1),95-101.
MLA Liang, XB,et al."Secretory expression of a heterologous nattokinase in Lactococcus lactis".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 75.1(2007):95-101.
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