The application of DGGE and AFLP-derived SCAR for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)
Zhang, JB; Wang, HJ; Cai, ZP; jbzhang30@vip.sina.com
2007
发表期刊FOOD CONTROL
ISSN0956-7135
卷号18期号:6页码:672-676
摘要In an attempt to authenticate commercial frauds in fresh products of Atlantic salmon (Salmo salai) by being adulterated and substituted with those of rainbow trout (Oncorhynchus mykiss), two molecular tools, AFLP (amplified fragment length polymorphism) and DGGE (denaturing gradient gel electrophoresis) were explored here. A species-specific SCAR (sequence characterized amplified region) marker for rainbow trout (O. mykiss) was developed based on AFLP analysis. In addition, DGGE was utilized to discriminate Atlantic salmon (S. salar) from rainbow trout employing a fragment of the mitochondrial cytochrome b gene. Analysis of experimental mixtures demonstrated that the detection sensitivity of AFLP-derived SCAR is higher than that of DGGE. In order to ascertain commercial frauds are fabricated by the adulteration or substitution of rainbow trout, DGGE analysis of cytochrome b gene should be combined because only one species-specific SCAR just for rainbow trout was obtained in this study. (c) 2006 Elsevier Ltd. All rights reserved.
部门归属Chinese Acad Sci, LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Grad Inst, Beijing 100049, Peoples R China
关键词Fraud Authentication Atlantic Salmon Rainbow Trout Aflp Scar Dgge
学科领域Food Science & Technology
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收录类别sci
资助项目LED
WOS记录号WOS:000244153400010
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5913
专题热带海洋环境国家重点实验室(LTO)
通讯作者jbzhang30@vip.sina.com
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Zhang, JB,Wang, HJ,Cai, ZP,et al. The application of DGGE and AFLP-derived SCAR for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)[J]. FOOD CONTROL,2007,18(6):672-676.
APA Zhang, JB,Wang, HJ,Cai, ZP,&jbzhang30@vip.sina.com.(2007).The application of DGGE and AFLP-derived SCAR for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss).FOOD CONTROL,18(6),672-676.
MLA Zhang, JB,et al."The application of DGGE and AFLP-derived SCAR for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)".FOOD CONTROL 18.6(2007):672-676.
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