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学科主题: Food Science & Technology
题名: The application of DGGE and AFLP-derived SCAR for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)
作者: Zhang, JB ; Wang, HJ ; Cai, ZP
通讯作者: jbzhang30@vip.sina.com
关键词: fraud authentication ; Atlantic salmon ; rainbow trout ; AFLP ; SCAR ; DGGE
刊名: FOOD CONTROL
发表日期: 2007
卷: 18, 期:6, 页:672-676
收录类别: sci
部门归属: Chinese Acad Sci, LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; Chinese Acad Sci, Grad Inst, Beijing 100049, Peoples R China
项目归属: LED
摘要: In an attempt to authenticate commercial frauds in fresh products of Atlantic salmon (Salmo salai) by being adulterated and substituted with those of rainbow trout (Oncorhynchus mykiss), two molecular tools, AFLP (amplified fragment length polymorphism) and DGGE (denaturing gradient gel electrophoresis) were explored here. A species-specific SCAR (sequence characterized amplified region) marker for rainbow trout (O. mykiss) was developed based on AFLP analysis. In addition, DGGE was utilized to discriminate Atlantic salmon (S. salar) from rainbow trout employing a fragment of the mitochondrial cytochrome b gene. Analysis of experimental mixtures demonstrated that the detection sensitivity of AFLP-derived SCAR is higher than that of DGGE. In order to ascertain commercial frauds are fabricated by the adulteration or substitution of rainbow trout, DGGE analysis of cytochrome b gene should be combined because only one species-specific SCAR just for rainbow trout was obtained in this study. (c) 2006 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000244153400010
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5913
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Recommended Citation:
Zhang, JB; Wang, HJ; Cai, ZP.The application of DGGE and AFLP-derived SCAR for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss),FOOD CONTROL,2007,18(6):672-676
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