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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians
作者: Zhu, L ; Song, LS ; Xu, W ; Qian, PY
通讯作者: boqianpy@ust.hk
关键词: C-type lectin ; CRD ; Argopecten irradians ; tissue distribution ; mRNA expression ; injury healing ; immune response
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2008
卷: 25, 期:3, 页:231-238
收录类别: sci
部门归属: [Zhu, Ling; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China; [Zhu, Ling; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Coastal Marine Lab, Hong Kong, Hong Kong, Peoples R China; [Zhu, Ling; Qian, Pei-Yuan] Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam LED, Guangzhou 510301, Guangdong, Peoples R China; [Song, Linsheng; Xu, Wei] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
项目归属: LED
摘要: C-type lectins are Ca2+-dependent carbohydrate-recognition proteins that play crucial roles in innate immunity. The cDNA of C-type lectin (AiCTL1) in the bay scallop Argopecten irradians was cloned by expressed sequence tag (EST) and RACE techniques. The full-length cDNA of AiCTL1 was 660 bp, consisting of a T-terminal. untranslated region (UTR) of 30 bp and a 3' UTR of 132 bp with a polyadenylation signal sequence AATAAA and a poly(A) tail. The AiCTL1 cDNA encoded a polypeptide of 166 amino acids with a putative signal peptide of 20 amino acid residues and a mature protein of 146 amino acids. The deduced amino acid sequence of AiCTL1 was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 121 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. AiCTL1 mRNA was dominantly expressed in the hemocytes of the bay scallop. The temporal expression of AiCTL1 mRNA in hemocytes was increased by 5.7-and 4.9-fold at 6 h after injury and 8 h after injection of bacteria, respectively. The structural features, high similarity and expression pattern of AiCTL1 indicate that the gene may be involved in injury heating and the immune response in A. irradians. (c) 2008 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000259759700006
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/5957
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Zhu, L; Song, LS; Xu, W; Qian, PY.Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians,FISH & SHELLFISH IMMUNOLOGY,2008,25(3):231-238
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