Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians
Zhu, L; Song, LS; Xu, W; Qian, PY; boqianpy@ust.hk
2008
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号25期号:3页码:231-238
摘要C-type lectins are Ca2+-dependent carbohydrate-recognition proteins that play crucial roles in innate immunity. The cDNA of C-type lectin (AiCTL1) in the bay scallop Argopecten irradians was cloned by expressed sequence tag (EST) and RACE techniques. The full-length cDNA of AiCTL1 was 660 bp, consisting of a T-terminal. untranslated region (UTR) of 30 bp and a 3' UTR of 132 bp with a polyadenylation signal sequence AATAAA and a poly(A) tail. The AiCTL1 cDNA encoded a polypeptide of 166 amino acids with a putative signal peptide of 20 amino acid residues and a mature protein of 146 amino acids. The deduced amino acid sequence of AiCTL1 was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 121 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. AiCTL1 mRNA was dominantly expressed in the hemocytes of the bay scallop. The temporal expression of AiCTL1 mRNA in hemocytes was increased by 5.7-and 4.9-fold at 6 h after injury and 8 h after injection of bacteria, respectively. The structural features, high similarity and expression pattern of AiCTL1 indicate that the gene may be involved in injury heating and the immune response in A. irradians. (c) 2008 Elsevier Ltd. All rights reserved.
部门归属[Zhu, Ling; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China; [Zhu, Ling; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Coastal Marine Lab, Hong Kong, Hong Kong, Peoples R China; [Zhu, Ling; Qian, Pei-Yuan] Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam LED, Guangzhou 510301, Guangdong, Peoples R China; [Song, Linsheng; Xu, Wei] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
关键词C-type Lectin Crd Argopecten Irradians Tissue Distribution Mrna Expression Injury Healing Immune Response
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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收录类别sci
资助项目LED
WOS记录号WOS:000259759700006
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5957
专题热带海洋环境国家重点实验室(LTO)
通讯作者boqianpy@ust.hk
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Zhu, L,Song, LS,Xu, W,et al. Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2008,25(3):231-238.
APA Zhu, L,Song, LS,Xu, W,Qian, PY,&boqianpy@ust.hk.(2008).Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,25(3),231-238.
MLA Zhu, L,et al."Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 25.3(2008):231-238.
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