Identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by Shewanella decolorationis S12
Hong, YG; Guo, J; Sun, GP; guopingsun@163.com
2008
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号80期号:3页码:517-524
摘要Shewanella decolorationis S12, a representative dissimilatory azo-reducing bacterium of Shewanella genus, can grow by coupling the oxidation of hydrogen to the reduction of azo compounds as the sole electron acceptor, indicating that an uptake hydrogenase is an important component for electron transfer for azoreduction. For searching to the uptake hydrogenase in the genome of S. decolorationis, two operons, hyd and hya, were cloned and sequenced, which encode periplasmically oriented Fe-only hydrogenase and a Ni-Fe hydrogenase, respectively, according to the homologous comparison with other bacterial hydrogenases. In order to assess the roles of these two enzymes in hydrogen-dependent azoreduction and growth, hyd- and hya-deficient mutants were generated by gene replacement. Hya was found to be required for hydrogen-dependent reduction of azo compound by resting cell suspensions and to be essential for growth with hydrogen as electron donor and azo compound as electron acceptor. Hyd, in contrast, was not. These findings suggest that Hya is an essential respiratory hydrogenase of dissimilatory azoreduction in S. decolorationis.
部门归属[Guo, Jun; Sun, Guo-Ping] Guangdong Inst Microbiol, Guangzhou 510070, Peoples R China; [Hong, Yi-Guo] Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam LED, Guangzhou 510301, Peoples R China
关键词Hydrogenase Dissimilatory Azoreduction Shewanella Decolorationis S12
学科领域Biotechnology & Applied Microbiology
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收录类别sci
资助项目LED
WOS记录号WOS:000258600300016
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/5979
专题热带海洋环境国家重点实验室(LTO)
通讯作者guopingsun@163.com
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Hong, YG,Guo, J,Sun, GP,et al. Identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by Shewanella decolorationis S12[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2008,80(3):517-524.
APA Hong, YG,Guo, J,Sun, GP,&guopingsun@163.com.(2008).Identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by Shewanella decolorationis S12.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,80(3),517-524.
MLA Hong, YG,et al."Identification of an uptake hydrogenase for hydrogen-dependent dissimilatory azoreduction by Shewanella decolorationis S12".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 80.3(2008):517-524.
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