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Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians
Zhu, L; Song, LS; Zhang, H; Zhao, JM; Li, CH; Xu, W; llingzhu@163.com; lshsong@ms.qdio.ac.cn
2008
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume35Issue:2Pages:125-132
AbstractApoptosis is an active process of cell death, which is an integral part of growth and development in multicellular organisms. The defender against cell death 1 (DAD1), the regulatory protein to inhibit the apoptosis process, was first cloned from the bay scallop Argopecten irradians by randomly sequencing a whole tissue cDNA library and rapid amplification of cDNA end (RACE). The full-length cDNA of the A. irradians DAD1 was 607 bp, consist of a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 205 bp with a canonical polyadenylation signal sequence AATAAA and a poly (A) tail, and an open reading frame of 339 bp. The deduced amino acid sequence of the A. irradians DAD1 showed 75.5% identity to Araneus ventricosus, 74.5% to Drosophila melanogaster, and 73.6% to Homo sapiens, Sus scrofa, Mesocricetus auratus, Rattus norvegicus and Mus musculus. Excluding the Saccharomyces cerevisiae DAD1 homologue, all animal DAD1 including A. irradians DAD1 homologue formed a subgroup and all plant DAD1 proteins formed another subgroup in the phylogenetic analysis. The A. irradians DAD1 was expressed in all examined tissues including adductor muscle, mantle, gills, digestive gland, gonad and hemolymph, suggesting that A. irradians DAD1 is expressed in most body tissues. Furthermore, the mRNA expression levels of A. irradians DAD1 gene of hemolymph were particularly high after injury, suggesting that the gene is responsive to injury stimuli.
Department[Zhu, Ling; Song, Linsheng; Zhang, Huan; Zhao, Jianmin; Li, Chenghua; Xu, Wei] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Zhu, Ling] Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Peoples R China
KeywordArgopecten Irradians Cdna Cloning Defender Against Apoptotic Cell Death (Dad1) Phylogeny Tissue Distribution Injury
Subject AreaBiochemistry & Molecular Biology
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Indexed Bysci
Funding ProjectLED
WOS IDWOS:000256082300007
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/6011
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorllingzhu@163.com; lshsong@ms.qdio.ac.cn
Recommended Citation
GB/T 7714
Zhu, L,Song, LS,Zhang, H,et al. Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):125-132.
APA Zhu, L.,Song, LS.,Zhang, H.,Zhao, JM.,Li, CH.,...&lshsong@ms.qdio.ac.cn.(2008).Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians.MOLECULAR BIOLOGY REPORTS,35(2),125-132.
MLA Zhu, L,et al."Molecular cloning and responsive expression to injury stimulus of a defender against cell death 1 (DAD1) gene from bay scallops Argopecten irradians".MOLECULAR BIOLOGY REPORTS 35.2(2008):125-132.
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