A novel serine protease with clip domain from scallop Chlamys farreri
Zhu, L; Song, LS; Mao, Y; Zhao, JM; Li, CH; Xu, W; llingzhu@163.com; lshsong@ms.qdio.ac.cn
2008
发表期刊MOLECULAR BIOLOGY REPORTS
ISSN0301-4851
卷号35期号:2页码:257-264
摘要The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of 1,152 bp, including a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 81 bp with a canonical polyadenylation signal sequence AATAAA and a poly(A) tail, and an open reading frame of 1,008 bp encoding a polypeptide of 336 amino acids with a putative signal peptide of 19 amino acids. The deduced amino acid sequence of Cf SP contained an amino-terminal clip domain with three disulfide bonds formed six conserved Cys residues, a carboxyl-terminal trypsin-like domain with the conserved His-Asp-Ser catalytic triad, and a low complexity linker sequence. The Cf SP was strongly expressed in hemocytes and the mRNA expression of Cf SP was up-regulated and increased 3.2-fold and 2.6-fold at 16 h after injection of Vibrio anguillarum and Micrococcus luteus. The results suggested that Cf SP gene might be involved in immune response of Gram-negative and Gram-positive microbial infection in scallop.
部门归属[Zhu, Ling; Song, Linsheng; Zhao, Jiangmin; Li, Chenghua; Xu, Wei] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Zhu, Ling] Chinese Acad Sci, Key Lab Trop Marine Environm Dynam LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; [Mao, Yuze] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Mariculture Ecol Div, Qingdao 266071, Peoples R China
关键词Chlamys Farreri Clip Domain Immune Response Mrna Expression Serine Protease Tissue Distribution
学科领域Biochemistry & Molecular Biology
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收录类别sci
资助项目LED
WOS记录号WOS:000256082300024
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/6013
专题热带海洋环境国家重点实验室(LTO)
通讯作者llingzhu@163.com; lshsong@ms.qdio.ac.cn
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Zhu, L,Song, LS,Mao, Y,et al. A novel serine protease with clip domain from scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):257-264.
APA Zhu, L.,Song, LS.,Mao, Y.,Zhao, JM.,Li, CH.,...&lshsong@ms.qdio.ac.cn.(2008).A novel serine protease with clip domain from scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,35(2),257-264.
MLA Zhu, L,et al."A novel serine protease with clip domain from scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 35.2(2008):257-264.
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