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学科主题: Biochemistry & Molecular Biology
题名: A novel serine protease with clip domain from scallop Chlamys farreri
作者: Zhu, L ; Song, LS ; Mao, Y ; Zhao, JM ; Li, CH ; Xu, W
通讯作者: llingzhu@163.com ; lshsong@ms.qdio.ac.cn
关键词: Chlamys farreri ; clip domain ; immune response ; mRNA expression ; serine protease ; tissue distribution
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2008
卷: 35, 期:2, 页:257-264
收录类别: sci
部门归属: [Zhu, Ling; Song, Linsheng; Zhao, Jiangmin; Li, Chenghua; Xu, Wei] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Zhu, Ling] Chinese Acad Sci, Key Lab Trop Marine Environm Dynam LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China; [Mao, Yuze] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Mariculture Ecol Div, Qingdao 266071, Peoples R China
项目归属: LED
摘要: The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of 1,152 bp, including a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 81 bp with a canonical polyadenylation signal sequence AATAAA and a poly(A) tail, and an open reading frame of 1,008 bp encoding a polypeptide of 336 amino acids with a putative signal peptide of 19 amino acids. The deduced amino acid sequence of Cf SP contained an amino-terminal clip domain with three disulfide bonds formed six conserved Cys residues, a carboxyl-terminal trypsin-like domain with the conserved His-Asp-Ser catalytic triad, and a low complexity linker sequence. The Cf SP was strongly expressed in hemocytes and the mRNA expression of Cf SP was up-regulated and increased 3.2-fold and 2.6-fold at 16 h after injection of Vibrio anguillarum and Micrococcus luteus. The results suggested that Cf SP gene might be involved in immune response of Gram-negative and Gram-positive microbial infection in scallop.
原文出处: 查看原文
WOS记录号: WOS:000256082300024
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6013
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Zhu, L; Song, LS; Mao, Y; Zhao, JM; Li, CH; Xu, W.A novel serine protease with clip domain from scallop Chlamys farreri,MOLECULAR BIOLOGY REPORTS,2008,35(2):257-264
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