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学科主题: Biochemical Research Methods
题名: Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa
作者: Mok, FSY ; Thiyagarajan, V ; Qian, PY
通讯作者: floramok@ust.hk ; rajan@hkucc.hku.hk ; boqianpy@ust.hk
刊名: PROTEOME SCIENCE
发表日期: 2009
卷: 7, 页:-
收录类别: sci
部门归属: [Mok, Flora S. Y.; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, KAUST Global Partnership Program, Dept Biol Atmospher Marine & Coastal Environm Pro, Hong Kong, Hong Kong, Peoples R China; [Thiyagarajan, Vengatesen] Univ Hong Kong, Sch Biol Sci, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China; [Qian, Pei-Yuan] Chinese Acad Sci, S China Sea Inst Oceanol, CAS SAFEA Int Partnership Program Creat Res Teams, Beijing 100864, Peoples R China
项目归属: LED
摘要: Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle. Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase). Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.
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WOS记录号: WOS:000273444500001
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6049
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Recommended Citation:
Mok, FSY; Thiyagarajan, V; Qian, PY.Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa,PROTEOME SCIENCE,2009,7():-
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文件名: Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.pdf
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