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Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa
Mok, FSY; Thiyagarajan, V; Qian, PY; floramok@ust.hk; rajan@hkucc.hku.hk; boqianpy@ust.hk
2009
Source PublicationPROTEOME SCIENCE
ISSN1477-5956
Volume7Pages:-
AbstractBackground: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle. Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase). Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.
Department[Mok, Flora S. Y.; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, KAUST Global Partnership Program, Dept Biol Atmospher Marine & Coastal Environm Pro, Hong Kong, Hong Kong, Peoples R China; [Thiyagarajan, Vengatesen] Univ Hong Kong, Sch Biol Sci, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China; [Qian, Pei-Yuan] Chinese Acad Sci, S China Sea Inst Oceanol, CAS SAFEA Int Partnership Program Creat Res Teams, Beijing 100864, Peoples R China
Subject AreaBiochemical Research Methods
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Indexed Bysci
Funding ProjectLED
WOS IDWOS:000273444500001
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/6049
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorfloramok@ust.hk; rajan@hkucc.hku.hk; boqianpy@ust.hk
Recommended Citation
GB/T 7714
Mok, FSY,Thiyagarajan, V,Qian, PY,et al. Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa[J]. PROTEOME SCIENCE,2009,7:-.
APA Mok, FSY,Thiyagarajan, V,Qian, PY,floramok@ust.hk,rajan@hkucc.hku.hk,&boqianpy@ust.hk.(2009).Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa.PROTEOME SCIENCE,7,-.
MLA Mok, FSY,et al."Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa".PROTEOME SCIENCE 7(2009):-.
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