Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress
Huang, GY; Wang, YS; huang_gyh@sina.com
2009
发表期刊CHEMOSPHERE
ISSN0045-6535
卷号77期号:7页码:1026-1029
摘要In this paper, we aimed to assess the roles of metallothioneins (MTs) in heavy metal tolerance by analyzing the expression level of BgM72 in leaves of Bruguiera gymnorrhiza in response to heavy metals. Eight-month-old B. gymnorrhiza seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 1, 3 and 7 d. A Real-time quantitative PCR protocol was developed to directly evaluate the expression of BgMT2, using 18S rRNA as a reference gene. Real-time quantitative PCR analysis demonstrated BgMT2 mRNA expression was regulated by Zn, Cu and Pb, but the regulation pattern was different for the three metals tested. Significant increase in the transcript level of BgM72 was also found in response to Zn, Cu and Pb in some experimental conditions. Our results confirm that BgMT2 gene is involved in the regulation of Zn, Cu and Pb in B. gymnorrhiza leaves. (C) 2009 Elsevier Ltd. All rights reserved.
部门归属[Huang, Guo-Yong; Wang, You-Shao] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China
关键词Copper Lead Metallothioneins Reverse Transcription Zinc
学科领域Environmental Sciences
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收录类别sci
资助项目LED
WOS记录号WOS:000271992200021
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被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/6065
专题热带海洋环境国家重点实验室(LTO)
通讯作者huang_gyh@sina.com
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Huang, GY,Wang, YS,huang_gyh@sina.com. Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress[J]. CHEMOSPHERE,2009,77(7):1026-1029.
APA Huang, GY,Wang, YS,&huang_gyh@sina.com.(2009).Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress.CHEMOSPHERE,77(7),1026-1029.
MLA Huang, GY,et al."Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress".CHEMOSPHERE 77.7(2009):1026-1029.
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