中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 热带海洋环境国家重点实验室(LTO)  > 期刊论文
学科主题: Environmental Sciences
题名: Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress
作者: Huang, GY ; Wang, YS
通讯作者: huang_gyh@sina.com
关键词: Copper ; Lead ; Metallothioneins ; Reverse transcription ; Zinc
刊名: CHEMOSPHERE
发表日期: 2009
卷: 77, 期:7, 页:1026-1029
收录类别: sci
部门归属: [Huang, Guo-Yong; Wang, You-Shao] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LED
摘要: In this paper, we aimed to assess the roles of metallothioneins (MTs) in heavy metal tolerance by analyzing the expression level of BgM72 in leaves of Bruguiera gymnorrhiza in response to heavy metals. Eight-month-old B. gymnorrhiza seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 1, 3 and 7 d. A Real-time quantitative PCR protocol was developed to directly evaluate the expression of BgMT2, using 18S rRNA as a reference gene. Real-time quantitative PCR analysis demonstrated BgMT2 mRNA expression was regulated by Zn, Cu and Pb, but the regulation pattern was different for the three metals tested. Significant increase in the transcript level of BgM72 was also found in response to Zn, Cu and Pb in some experimental conditions. Our results confirm that BgMT2 gene is involved in the regulation of Zn, Cu and Pb in B. gymnorrhiza leaves. (C) 2009 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000271992200021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6065
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Expression analysis of type 2 metallothionein gene in mangrove species.pdf(223KB)----开放获取--View Download

Recommended Citation:
Huang, GY; Wang, YS.Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress,CHEMOSPHERE,2009,77(7):1026-1029
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Huang, GY]'s Articles
[Wang, YS]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Huang, GY]‘s Articles
[Wang, YS]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Expression analysis of type 2 metallothionein gene in mangrove species.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace