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学科主题: Biochemistry & Molecular Biology
题名: Identification of a C-type lectin from the bay scallop Argopecten irradians
作者: Zhu, L ; Song, LS ; Xu, W ; Qian, PY
通讯作者: llingzhu@163.com ; boqianpy@ust.hk
关键词: C-type lectin ; CRD ; Argopecten irradians ; Tissue distribution ; mRNA expression ; Immune response
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2009
卷: 36, 期:5, 页:1167-1173
收录类别: sci
部门归属: [Zhu, Ling; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China; [Zhu, Ling; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China; [Zhu, Ling; Qian, Pei-Yuan] Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China; [Song, Linsheng; Xu, Wei] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
项目归属: LED
摘要: C-type lectins are Ca2+ dependent carbohydrate-recognition proteins that play crucial roles in the invertebrate innate immunity, such as nonself recognition, activation of proPO system, antibacterial activity, promotion of phagocytosis and nodule formation. In this study, a novel C-type lectin of bay scallops Argopecten irradians (Ai Lec) was identified using expressed sequence tag (EST) and RACE techniques. The Ai Lec cDNA encoded a polypeptide of 171 amino acids with a putative signal peptide of 21 amino acid residues and a mature protein of 150 amino acids. The deduced amino acid sequence of Ai Lec was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 131 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. The expression of Ai Lec transcript was dominantly detected in the hepatopancreas and slightly detected in the haemocytes of normal scallops. 6 h after Vibrio anguillarum-challenge and 8 h after Micrococcus luteus-challenge, the temporal expression of Ai Lec mRNA in hemocytes was increased by 4.4- and 3.6-folds, respectively. The results suggested that Ai Lec was a constitutive and inducible acute-phase protein and might be involved in immune response to Gram-negative and Gram-positive microbial infection in bay scallop A. irradians.
原文出处: 查看原文
WOS记录号: WOS:000264851600042
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6129
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Zhu, L; Song, LS; Xu, W; Qian, PY.Identification of a C-type lectin from the bay scallop Argopecten irradians,MOLECULAR BIOLOGY REPORTS,2009,36(5):1167-1173
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