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学科主题: Chemistry, Analytical; Electrochemistry
题名: Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate
作者: Ding, JW ; Qin, W
通讯作者: wqin@yic.ac.cn
关键词: Polymer membrane electrodes ; Controlled-release ; Butyrylcholinesterase ; Pesticides ; Biosensors ; Membranes
刊名: ELECTROANALYSIS
发表日期: 2009
卷: 21, 期:17-18, 页:2030-2035
收录类别: sci
部门归属: [Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin, Wei] Yantai Univ, Sch Oceanol, Yantai 264005, Peoples R China
项目归属: LED
摘要: A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).
原文出处: 查看原文
WOS记录号: WOS:000270399100020
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6165
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Recommended Citation:
Ding, JW; Qin, W.Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate,ELECTROANALYSIS,2009,21(17-18):2030-2035
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文件名: Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate.pdf
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