Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate
Ding, JW; Qin, W; wqin@yic.ac.cn
2009
发表期刊ELECTROANALYSIS
ISSN1040-0397
卷号21期号:17-18页码:2030-2035
摘要A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).
部门归属[Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Environm Chem Lab, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ding, Jiawang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin, Wei] Yantai Univ, Sch Oceanol, Yantai 264005, Peoples R China
关键词Polymer Membrane Electrodes Controlled-release Butyrylcholinesterase Pesticides Biosensors Membranes
学科领域Chemistry, Analytical ; Electrochemistry
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收录类别sci
资助项目LED
WOS记录号WOS:000270399100020
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/6165
专题热带海洋环境国家重点实验室(LTO)
通讯作者wqin@yic.ac.cn
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GB/T 7714
Ding, JW,Qin, W,wqin@yic.ac.cn. Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate[J]. ELECTROANALYSIS,2009,21(17-18):2030-2035.
APA Ding, JW,Qin, W,&wqin@yic.ac.cn.(2009).Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate.ELECTROANALYSIS,21(17-18),2030-2035.
MLA Ding, JW,et al."Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate".ELECTROANALYSIS 21.17-18(2009):2030-2035.
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