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学科主题: Marine & Freshwater Biology; Toxicology
题名: Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress
作者: Huang, GY ; Wang, YS
通讯作者: huang_gyh@sina.com
关键词: Metals ; GST fusion protein ; Metallothionein ; Reverse transcription
刊名: AQUATIC TOXICOLOGY
发表日期: 2010
卷: 99, 期:1, 页:86-92
收录类别: sci
部门归属: [Huang, Guo-Yong; Wang, You-Shao] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Guo-Yong] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China
项目归属: LED
摘要: Metallothioneins (MTs) are a family of low-molecular-weight cysteine-rich proteins and are thought to play possible roles in metal metabolism or detoxification. To evaluate the roles of metallothioneins in metal homeostasis or tolerance in Avicennia marina, a real-time quantitative PCR protocol was developed to directly evaluate the expression of AmMT2 mRNA, when A. marina seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 3 and 7d. Real-time quantitative PCR results indicated that the regulation of AmMT2 mRNA expression by Zn, Cu and Pb was strongly dependent on concentration and time of exposure. A significant increase in the transcripts of AmMT2 gene was also found in response to Zn, Cu and Pb, at least under some experimental conditions. When AmMT2 was overexpressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST), the transgenic bacteria showed an increased tolerance to Zn, Cu, Pb and Cd exposure as compared to control strains. Moreover, GST-AmMT2 was purified from E. coli cells grown in the presence of 400 mu M Zn, Cu, Pb or Cd. The purified GST-AmMT2 fusion protein could bind higher levels of all four metals than GST alone. Taken together, these data support the hypothesis that AmMT2 may be involved in processes of metal homeostasis or tolerance in A. marina. (C) 2010 Elsevier B.V. All rights reserved.
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WOS记录号: WOS:000279277800009
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6283
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Huang, GY; Wang, YS.Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress,AQUATIC TOXICOLOGY,2010,99(1):86-92
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