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Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress
Huang, GY; Wang, YS; huang_gyh@sina.com
2010
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume99Issue:1Pages:86-92
AbstractMetallothioneins (MTs) are a family of low-molecular-weight cysteine-rich proteins and are thought to play possible roles in metal metabolism or detoxification. To evaluate the roles of metallothioneins in metal homeostasis or tolerance in Avicennia marina, a real-time quantitative PCR protocol was developed to directly evaluate the expression of AmMT2 mRNA, when A. marina seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 3 and 7d. Real-time quantitative PCR results indicated that the regulation of AmMT2 mRNA expression by Zn, Cu and Pb was strongly dependent on concentration and time of exposure. A significant increase in the transcripts of AmMT2 gene was also found in response to Zn, Cu and Pb, at least under some experimental conditions. When AmMT2 was overexpressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST), the transgenic bacteria showed an increased tolerance to Zn, Cu, Pb and Cd exposure as compared to control strains. Moreover, GST-AmMT2 was purified from E. coli cells grown in the presence of 400 mu M Zn, Cu, Pb or Cd. The purified GST-AmMT2 fusion protein could bind higher levels of all four metals than GST alone. Taken together, these data support the hypothesis that AmMT2 may be involved in processes of metal homeostasis or tolerance in A. marina. (C) 2010 Elsevier B.V. All rights reserved.
Department[Huang, Guo-Yong; Wang, You-Shao] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Guo-Yong] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China
KeywordMetals Gst Fusion Protein Metallothionein Reverse Transcription
Subject AreaMarine & Freshwater Biology ; Toxicology
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Indexed Bysci
Funding ProjectLED
WOS IDWOS:000279277800009
Citation statistics
Cited Times:55[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/6283
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorhuang_gyh@sina.com
Recommended Citation
GB/T 7714
Huang, GY,Wang, YS,huang_gyh@sina.com. Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress[J]. AQUATIC TOXICOLOGY,2010,99(1):86-92.
APA Huang, GY,Wang, YS,&huang_gyh@sina.com.(2010).Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress.AQUATIC TOXICOLOGY,99(1),86-92.
MLA Huang, GY,et al."Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to metal stress".AQUATIC TOXICOLOGY 99.1(2010):86-92.
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