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学科主题: Biotechnology & Applied Microbiology
题名: A comparison of primer sets for detecting 16S rRNA and hydrazine oxidoreductase genes of anaerobic ammonium-oxidizing bacteria in marine sediments
作者: Li, M ; Hong, YG ; Klotz, MG ; Gu, JD
通讯作者: jdgu@hkucc.hku.hk
关键词: Anammox bacteria ; Primer sets ; 16S RNA gene ; hzo gene ; Marine sediments
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2010
卷: 86, 期:2, 页:781-790
收录类别: sci
部门归属: [Li, Meng; Gu, Ji-Dong] Univ Hong Kong, Sch Biol Sci, Div Microbiol, Hong Kong, Hong Kong, Peoples R China; [Hong, Yiguo] Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam LED, Guangzhou 510301, Guangdong, Peoples R China; [Klotz, Martin Gunter] Univ Louisville, Dept Biol Microbiol & Immunol, Louisville, KY 40292 USA; [Gu, Ji-Dong] Univ Hong Kong, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China
项目归属: LED
摘要: Published polymerase chain reaction primer sets for detecting the genes encoding 16S rRNA gene and hydrazine oxidoreductase (hzo) in anammox bacteria were compared by using the same coastal marine sediment samples. While four previously reported primer sets developed to detect the 16S rRNA gene showed varying specificities between 12% and 77%, an optimized primer combination resulted in up to 98% specificity, and the recovered anammox 16S rRNA gene sequences were > 95% sequence identical to published sequences from anammox bacteria in the Candidatus "Scalindua" group. Furthermore, four primer sets used in detecting the hzo gene of anammox bacteria were highly specific (up to 92%) and efficient, and the newly designed primer set in this study amplified longer hzo gene segments suitable for phylogenetic analysis. The optimized primer set for the 16S rRNA gene and the newly designed primer set for the hzo gene were successfully applied to identify anammox bacteria from marine sediments of aquaculture zone, coastal wetland, and deep ocean where the three ecosystems form a gradient of anthropogenic impact. Results indicated a broad distribution of anammox bacteria with high niche-specific community structure within each marine ecosystem.
原文出处: 查看原文
WOS记录号: WOS:000274707600038
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/6321
Appears in Collections:热带海洋环境动力实验室(LTO)_期刊论文

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Li, M; Hong, YG; Klotz, MG; Gu, JD.A comparison of primer sets for detecting 16S rRNA and hydrazine oxidoreductase genes of anaerobic ammonium-oxidizing bacteria in marine sediments,APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,86(2):781-790
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文件名: A comparison of primer sets for detecting 16S rRNA and hydrazine oxidoreductase genes of anaerobic ammonium-oxidizing bacteria in marine sediments .pdf
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