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题名: 海水养殖真鲷弧菌病病原菌外毒素的点酶法检测
作者: 吴后波 ; 潘金培
关键词: 海水养殖 ; 真鲷 ; 弧菌病 ; 病原菌 ; 外毒素 ; 点酶法 ; 检测技术
刊名: 水产学报
发表日期: 2003-01-01
卷: 27, 期:6, 页:606-609
部门归属: 中国科学院南海海洋研究所,广东广州510301
项目归属: 海洋生物
摘要: Vibriosis, caused by Vibrio mimicus, became a major cause of serious economic losses in the farming of red sea bream along the coast of southern China. It has been demonstrated that the pathogenicity of V. mimicus for red sea bream is due to the production of a heat-labile exotoxin. In the present paper, a dot immunobinding assay (DIA) to detect the toxin produced by V. mimicus was developed. The lowest concentration of the toxin the DIA could detect was 0. 025/μg·mL^-1 The DIA could detect the toxin in the supernatant of liver and spleen of the diseased red sea bream Pagrus major, even if the supernatant was diluted by 1000 times. It was concluded that the DIA would be useful for rapid and effective detection of the toxin in large samples.
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/7415
Appears in Collections:广东省海洋药物重点实验室_期刊论文

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Recommended Citation:
吴后波;潘金培 .海水养殖真鲷弧菌病病原菌外毒素的点酶法检测,水产学报,2003,27(6):606-609
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文件名: 海水养殖真鲷弧菌病病原菌外毒素的点酶法检测.pdf
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