Molecular identification of potential pathogens in water and air of a hospital therapy pool
Angenent, LT; Kelley, ST; St Amand, A; Pace, NR; Hernandez, MT; angenent@seas.wustl.edu
2005
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
卷号102期号:13页码:4860-4865
摘要Indoor warm-water therapy pool workers in a Midwestern regional hospital were diagnosed with non-tuberculosis pulmonary hypersensitive pneumonitis and Mycobacterium avium infections. In response, we conducted a multiseason survey of microorganisms present in this therapy pool water, in biofilms associated with the pool containment walls, and in air immediately above the pool. The survey used culture, microscopy, and culture-independent molecular phylogenetic analyses. Although outfitted with a state-of-theart UV-peroxide disinfection system, the numbers of bacteria in the therapy pool water were relatively high compared with the potable water used to fill the pool. Regardless of the source, direct microscopic counts of microbes were routinely approximate to 1,000 times greater than conventional plate counts. Analysis of clone libraries of small subunit rRNA genes from environmental DNA provided phylogenetic diversity estimates of the microorganisms collected in and above the pool. A survey of >11,300 rRNA genes yielded a total of 628 unique sequences, the most common of which was nearly identical to that of M. avium strains. The high proportion of clones with different Mycobacterium spp. rRNA genes suggested that such organisms comprised a significant fraction of microbes in the pool water (to >30%) and preferentially partition into aerosols (to >80%) relative to other waterborne bacteria present. The results of the study strongly validate aerosol partitioning as a mechanism for disease transfer in these environments. The results also show that culture protocols currently used by public health facilities and agencies are seriously inadequate for the detection and enumeration of potential pathogens.
部门归属Washington Univ, Environm Engn Sci Program, St Louis, MO 63130 USA; Washington Univ, Dept Chem Engn, St Louis, MO 63130 USA; San Diego State Univ, Dept Biol, San Diego, CA 92182 USA; Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA; Univ Colorado, Dept Civil Environm & Architectural Engn, Boulder, CO 80309 USA
关键词16s Rrna Genes Bioaerosols Hypersensitive Pneumonitis Molecular Survey Mycobacterium
学科领域Multidisciplinary Sciences
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收录类别sci
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/8479
专题海洋科技动态信息-印太暖池系统研究
通讯作者angenent@seas.wustl.edu
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Angenent, LT,Kelley, ST,St Amand, A,et al. Molecular identification of potential pathogens in water and air of a hospital therapy pool[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2005,102(13):4860-4865.
APA Angenent, LT,Kelley, ST,St Amand, A,Pace, NR,Hernandez, MT,&angenent@seas.wustl.edu.(2005).Molecular identification of potential pathogens in water and air of a hospital therapy pool.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,102(13),4860-4865.
MLA Angenent, LT,et al."Molecular identification of potential pathogens in water and air of a hospital therapy pool".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 102.13(2005):4860-4865.
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