中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biochemistry & Molecular Biology; Chemistry, Medicinal
题名: Identification of the Biosynthetic Gene Cluster and Regulatory Cascade for the Synergistic Antibacterial Antibiotics Griseoviridin and Viridogrisein in Streptomyces griseoviridis
其他题名: mannual of IR1
作者: Xie, YC ; Wang, B ; Liu, J ; Zhou, JC ; Ma, JY ; Huang, HB ; Ju, JH
通讯作者: jju@scsio.ac.cn
关键词: antibacterial ; biosynthetic gene clusters ; griseoviridin ; regulatory cascades ; Streptomyces griseoviridis ; viridogrisein
刊名: CHEMBIOCHEM
发表日期: 2012
卷: 13, 期:18, 页:2745-2757
收录类别: sci
合作性质: 填入合作性质等
部门归属: [Xie, Yunchang; Wang, Bo; Liu, Jing; Zhou, Junchao; Ma, Junying; Huang, Hongbo; Ju, Jianhua] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Xie, Yunchang; Liu, Jing; Zhou, Junchao] Chinese Acad Sci, Grad Univ, Beijing 110039, Peoples R China
项目归属: LMB
产权排名: 产权排序
资助者: 国科图
摘要: Griseoviridin (GV) and viridogrisein (VG, also referred to as etamycin), produced by Streptomyces griseoviridis, are two chemically unrelated compounds belonging to the streptogramin family. Both of these natural products demonstrate broad-spectrum antibacterial activity and constitute excellent candidates for future drug development. To elucidate the biosynthetic machinery associated with production of these two unique antibiotics, the gene cluster responsible for both GV and VG production was identified within the Streptomyces griseoviridis genome and characterized, and its function in GV and VG biosynthesis was confirmed by inactivation of 30 genes and complementation experiments. This sgv gene cluster is localized to a 105 kb DNA region that consists of 36 open reading frames (ORFs), including four nonribosomal peptide synthetases (NRPSs) for VG biosynthesis and a set of hybrid polyketide synthases (PKS)-NRPSs with a discrete acyltransferase (AT), SgvQ, to assemble the GV backbone. The enzyme encoding genes for VG versus GV biosynthesis are separated into distinct halves of the cluster. A series of four genes: sgvA, sgvB, sgvC, and sgvK, were found downstream of the PKS-NRPS; these likely code for construction of a ?-butyrolactone (GBL)-like molecule. GBLs and the corresponding GBL receptor systems are the highest ranked regulators that are able to coordinate the two streptomyces antibiotic regulatory protein (SARP) family positive regulators SgvR2 and SgvR3; both are key biosynthetic activators. Models of GV, VG, and GBL biosynthesis were proposed by using functional gene assignments, determined on the basis of bioinformatics analysis and further supported by in vivo gene inactivation experiments. Overall, this work provides new insights into the biosyntheses of the GV and VG streptogramins that are potentially applicable to a host of combinatorial biosynthetic scenarios.
英文摘要: description.abstract
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000312546300015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9726
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Identification of the Biosynthetic Gene Cluster and Regulatory.pdf(1234KB)----开放获取--View Download

Recommended Citation:
Xie, YC; Wang, B; Liu, J; Zhou, JC; Ma, JY; Huang, HB; Ju, JH.Identification of the Biosynthetic Gene Cluster and Regulatory Cascade for the Synergistic Antibacterial Antibiotics Griseoviridin and Viridogrisein in Streptomyces griseoviridis,CHEMBIOCHEM,2012,13(18):2745-2757
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xie, YC]'s Articles
[Wang, B]'s Articles
[Liu, J]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xie, YC]‘s Articles
[Wang, B]‘s Articles
[Liu, J]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification of the Biosynthetic Gene Cluster and Regulatory.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace