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An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua
Li, J; Zhao, GZ; Varma, A; Qin, S; Xiong, Z; Huang, HY; Zhu, WY; Zhao, LX; Xu, LH; Zhang, S; Li, WJ; wjli@ynu.edu.cn
2012
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:12Pages:-
AbstractEndophytic actinobacteria colonize internal tissues of their host plants and are considered as a rich and reliable source of diverse species and functional microorganisms. In this study, endophytic actinobacterial strain YIM 63111 was isolated from surface-sterilized tissue of the medicinal plant Artemisia annua. We identified strain YIM 63111 as a member of the genus Pseudonocardia. A. annua seedlings grown under both sterile and greenhouse conditions were inoculated with strain YIM 63111. The growth of A. annua seedlings was strongly reduced when YIM 63111 was inoculated at higher concentrations under sterile conditions. However, no growth inhibition was observed when A. annua was grown under greenhouse conditions. Using an enhanced green fluorescent protein (EGFP) expressing YIM 63111 strain, we also observed the endophytic colonization of A. annua seedling using confocal laser-scanning microscopy. The transcription levels of the key genes involved in artemisinin biosynthesis were investigated using real time RT-PCR, revealing that cytochrome P450 monooxygenase (CYP71AV1) and cytochrome P450 oxidoreductase (CPR) expression were up-regulated in A. annua upon inoculation with strain YIM 63111 under certain conditions. The up-regulation of these genes was associated with the increased accumulation of artemisinin. These results suggest that endophytic actinobacteria effectively stimulate certain plant defense responses. Our data also demonstrate the use of Pseudonocardia sp. strain YIM 63111 as a promising means to enhance artemisinin production in plants.
Department[Li, Jie; Zhao, Guo-Zhen; Huang, Hai-Yu; Zhu, Wen-Yong; Zhao, Li-Xing; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Key Lab Microbial Divers SW China, Minist Educ, Kunming, Peoples R China; [Li, Jie; Zhao, Guo-Zhen; Huang, Hai-Yu; Zhu, Wen-Yong; Zhao, Li-Xing; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Yunnan Inst Microbiol, Kunming, Peoples R China; [Li, Jie; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresource Sustainable Utilizat C, Guangdong Key Lab Marine Mat Med, S China Sea Inst Oceanol,RNAM Ctr Marine Microbio, Guangzhou, Guangdong, Peoples R China; [Varma, Ajit] Amity Univ Uttar Pradesh, Amity Inst Microbial Technol, Noida, India; [Qin, Sheng] Jiangsu Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou, Peoples R China; [Xiong, Zhi] SW Forestry Univ, Sch Forestry Resources, Kunming, Peoples R China; [Li, Wen-Jun] Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land CAS, Xinjiang Inst Ecol & Geog, Urumqi, Peoples R China
Subject AreaMultidisciplinary Sciences
Language英语
Funding ProjectLMB
WOS IDWOS:000313236200103
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9730
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorwjli@ynu.edu.cn
Recommended Citation
GB/T 7714
Li, J,Zhao, GZ,Varma, A,et al. An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua[J]. PLOS ONE,2012,7(12):-.
APA Li, J.,Zhao, GZ.,Varma, A.,Qin, S.,Xiong, Z.,...&wjli@ynu.edu.cn.(2012).An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua.PLOS ONE,7(12),-.
MLA Li, J,et al."An Endophytic Pseudonocardia Species Induces the Production of Artemisinin in Artemisia annua".PLOS ONE 7.12(2012):-.
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