A unique tRNA gene family and a novel, highly expressed ORF in the mitochondrial genome of the silver-lip pearl oyster, Pinctada maxima (Bivalvia: Pteriidae)
Wu, XY; Li, XL; Li, L; Yu, ZN; carlzyu@scsio.ac.cn
2012
发表期刊GENE
ISSN0378-1119
卷号510期号:1页码:22-31
摘要Characteristics of mitochondrial (mt) DNA such as gene content and arrangement, as well as mt tRNA secondary structure, are frequently used in comparative genomic analyses because they provide valuable phylogenetic information. However, most analyses do not characterize the relationship of tRNA genes from the same mt genome and, in some cases, analyses overlook possible novel open reading frames (ORFs) when the 13 expected protein-coding genes are already annotated. In this study, we describe the sequence and characterization of the complete mt genome of the silver-lip pearl oyster, Pinctada maxima. The 16,994-bp mt genome contains the same 13 protein-coding genes (PCGs) and two ribosomal RNA genes typical of metazoans. The gene arrangement, however, is completely distinct from that of all other available bivalve mt genomes, and a unique tRNA gene family is observed in this genome. The unique tRNA gene family includes two trnS(-AGY) and trnQ genes, a trnM isomerism, but it lacks trnS(-CUN). We also report the first clear evidence of alloacceptor tRNA gene recruitment (trnP-->trnS(-AGY)) in mollusks. In addition, a novel ORF (orfUR1) expressed at high levels is present in the mt genome of this pearl oyster. This gene contains a conserved domain, "Oxidored_q1_N", which is a member of Complex I and thus may play an important role in key biological functions. Because orfUR1 has a very similar nucleotide composition and codon bias to that of other genes in this genome, we hypothesize that this gene may have been moved to the mt genome via gene transfer from the nuclear genome at an early stage of speciation of P. maxima, or it may have evolved as a result of gene duplication, followed by rapid sequence divergence. Lastly, a 319-bp region was identified as the possible control region (CR) even though it does not correspond to the longest non-coding region in the genome. Unlike other studies of mt genomes, this study compares the evolutionary patterns of all available bivalve mt tRNA and 148 genes. (C) 2012 Elsevier B.V. All rights reserved.
部门归属[Wu, Xiangyun; Li, Xiaoling; Li, Lu; Yu, Ziniu] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Appl Marine Biol, Key Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Li, Xiaoling] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
关键词Pinctada Maxima Mitochondrial Genome Pearl Oyster Trna Gene Recruitment Atp8 Control Region
学科领域Genetics & Heredity
语种英语
资助项目LMB
WOS记录号WOS:000313611700004
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9744
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Wu, XY,Li, XL,Li, L,et al. A unique tRNA gene family and a novel, highly expressed ORF in the mitochondrial genome of the silver-lip pearl oyster, Pinctada maxima (Bivalvia: Pteriidae)[J]. GENE,2012,510(1):22-31.
APA Wu, XY,Li, XL,Li, L,Yu, ZN,&carlzyu@scsio.ac.cn.(2012).A unique tRNA gene family and a novel, highly expressed ORF in the mitochondrial genome of the silver-lip pearl oyster, Pinctada maxima (Bivalvia: Pteriidae).GENE,510(1),22-31.
MLA Wu, XY,et al."A unique tRNA gene family and a novel, highly expressed ORF in the mitochondrial genome of the silver-lip pearl oyster, Pinctada maxima (Bivalvia: Pteriidae)".GENE 510.1(2012):22-31.
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