Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement
He, LS; Xu, Y; Matsumura, K; Zhang, Y; Zhang, G; Qi, SH; Qian, PY; boqianpy@ust.hk
2012
发表期刊PLOS ONE
ISSN1932-6203
卷号7期号:10页码:e47195
摘要The barnacle Balanus (= Amphibalanus) amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK) from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK) was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.
部门归属[He, Li-Sheng; Xu, Ying; Matsumura, Kiyotaka; Zhang, Yu; Zhang, Gen; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, KAUST Global Collaborat Res Program, Div Life Sci, Kowloon, Hong Kong Sar, Peoples R China; [Qi, Shu-Hua] Chinese Acad Sci, S China Sea Inst Oceanog, Guangzhou, Guangdong, Peoples R China
学科领域Multidisciplinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000310310200029
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9764
专题中科院海洋生物资源可持续利用重点实验室
通讯作者boqianpy@ust.hk
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He, LS,Xu, Y,Matsumura, K,et al. Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement[J]. PLOS ONE,2012,7(10):e47195.
APA He, LS.,Xu, Y.,Matsumura, K.,Zhang, Y.,Zhang, G.,...&boqianpy@ust.hk.(2012).Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement.PLOS ONE,7(10),e47195.
MLA He, LS,et al."Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement".PLOS ONE 7.10(2012):e47195.
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