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学科主题: Environmental Sciences; Marine & Freshwater Biology
题名: Analysis of Pacific oyster larval proteome and its response to high-CO2
作者: Dineshram, R ; Wong, KKW ; Xiao, S ; Yu, ZN ; Qian, PY ; Thiyagarajan, V
通讯作者: rajan@hku.hk
关键词: Environmental proteomics ; High-CO2 ; Larval proteome ; Ocean acidification ; Pacific oyster ; Veliger
刊名: MARINE POLLUTION BULLETIN
发表日期: 2012
卷: 64, 期:10, 页:2160-2167
部门归属: [Dineshram, R.; Wong, Kelvin K. W.; Thiyagarajan, Vengatesen] Univ Hong Kong, Swire Inst Marine Sci, Hong Kong, Hong Kong, Peoples R China; [Dineshram, R.; Wong, Kelvin K. W.; Thiyagarajan, Vengatesen] Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China; [Xiao, Shu; Yu, Ziniu] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Qian, Pei Yuan] Univ Hong Kong, Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
项目归属: LMB
摘要: Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. (C) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
WOS记录号: WOS:000310929500035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9776
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Dineshram, R; Wong, KKW; Xiao, S; Yu, ZN; Qian, PY; Thiyagarajan, V.Analysis of Pacific oyster larval proteome and its response to high-CO2,MARINE POLLUTION BULLETIN,2012,64(10):2160-2167
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文件名: Analysis of Pacific oyster larval proteome and its response.pdf
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