Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)
Wei, JG; Guo, ML; Ji, HS; Yan, Y; Ouyang, ZL; Huang, XH; Hang, YH; Qin, QW; qinqw@scsio.ac.cn
2012
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号33期号:4页码:916-925
摘要Translationally controlled tumor protein (TCTP) is an important molecule involved in multiple biological processes, such as cell growth, cell cycle progression, malignant transformation, and enhancement of the anti-apoptotic activity. In this study, the TCTP from orange-spotted grouper Epinephelus coioides (Ec-TCTP) was cloned and characterized. The full-length cDNA of Ec-TCTP was comprised of 1057 bp with a 510 bp open reading frame that encodes a putative protein of 170 amino acids. Recombinant Ec-TCTP (rEc-TCTP) was expressed in Escherichia BL21 (DE3) and purified for mouse anti-Ec-TCTP serum preparation. The rEc-TCTP fusion protein was demonstrated to possess antioxidant activity, which conferred resistance to H2O2 damage. Quantitative real-time PCR analysis revealed that Ec-TCTP mRNA is predominately expressed in the liver, and the expression was up-regulated in the liver of grouper after viral challenge with Singapore grouper iridovirus (SGIV). Intracellular localization revealed that Ec-TCTP expression was distributed predominantly in the cytoplasm. Although human TCTP has a role in apoptosis regulation, it is not known if grouper TCTP has any role in apoptosis regulation. Strikingly, grouper TCTP, when overexpressed in fathead minnow (FHM) cells, protected them from cell death induced by cycloheximide (CHX). In addition, overexpressed Ec-TCTP in grouper spleen (GS) cells inhibited the replication of SGIV. These results suggest that Ec-TCTP may play a critical role in their response to SGIV infection, through regulation of a cell death pathway that is common to fish and humans. (C) 2012 Elsevier Ltd. All rights reserved.
部门归属[Wei, Jingguang; Guo, Minglan; Yan, Yang; Ouyang, Zhengliang; Huang, Xiaohong; Hang, Youhua; Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
关键词Translationally Controlled Tumor Protein (Tctp) Epinephelus Coioides Sgiv Intracellular Localization Viral Replication
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000310185800028
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9784
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Wei, JG,Guo, ML,Ji, HS,et al. Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):916-925.
APA Wei, JG.,Guo, ML.,Ji, HS.,Yan, Y.,Ouyang, ZL.,...&qinqw@scsio.ac.cn.(2012).Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV).FISH & SHELLFISH IMMUNOLOGY,33(4),916-925.
MLA Wei, JG,et al."Grouper translationally controlled tumor protein prevents cell death and inhibits the replication of Singapore grouper iridovirus (SGIV)".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):916-925.
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