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学科主题: Environmental Sciences; Microbiology
题名: Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods
作者: Qin, S ; Chen, HH ; Zhao, GZ ; Li, J ; Zhu, WY ; Xu, LH ; Jiang, JH ; Li, WJ
通讯作者: wjli@ynu.edu.cn
刊名: ENVIRONMENTAL MICROBIOLOGY REPORTS
发表日期: 2012
卷: 4, 期:5, 页:522-531
部门归属: [Qin, Sheng; Zhao, Guo-Zhen; Zhu, Wen-Yong; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Minist Educ, Key Lab Microbial Divers SW China, Kunming 650091, Yunnan, Peoples R China; [Qin, Sheng; Zhao, Guo-Zhen; Zhu, Wen-Yong; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Yunnan Inst Microbiol, Lab Conservat & Utilizat Bioresources, Kunming 650091, Yunnan, Peoples R China; [Qin, Sheng; Jiang, Ji-Hong] Jiangsu Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China; [Chen, Hua-Hong] Chuxiong Normal Coll, Dept Chem, Chuxiong 675000, Yunnan, Peoples R China; [Li, Jie] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,S China Sea Inst, Guangzhou 510301, Guangdong, Peoples R China; [Li, Wen-Jun] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Xinjiang, Peoples R China
项目归属: LMB
摘要: Endophytes are now considered as an important component of biodiversity. However, the diversity of endophytic actinobacteria associated with tropical rainforest native medicinal plants is essentially unknown. In this study, the diversity of endophytic actinobacteria residing in root, stem and leaf tissues of medicinal plant Maytenus austroyunnanensis collected from tropical rainforest in Xishuangbanna, China was investigated with a combination of cultivation and culture-independent analysis on the basis of 16S rRNA gene sequencing. By using different selective isolation media and methods, a total of 312 actinobacteria were obtained, and they were affiliated with the order Actinomycetales (distributed into 21 genera). Based on a protocol for endophytes enrichment, three 16S rRNA gene clone libraries were constructed and 84 distinct operational taxonomic units were identified and they distributed among the orders Actinomycetales and Acidimicrobiales, including eight suborders and at least 38 genera with a number of rare actinobacteria genera. Phylogenetic analysis showed that 32% of the clones in the libraries had lower than 97% similarities with related type strains. Interestingly, six genera from the order Actinomycetales and uncultured clones from Acidimicrobiales have not, to our knowledge, been previously reported as endophytes. Our study confirms abundant endophytic actinobacterial consortium in tropical rainforest native plant and suggests that this special habitat represents an underexplored reservoir of diverse and novel actinobacteria of potential interest for bioactive compounds discovery.
语种: 英语
WOS记录号: WOS:000309445800008
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9786
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Qin, S; Chen, HH; Zhao, GZ; Li, J; Zhu, WY; Xu, LH; Jiang, JH; Li, WJ.Abundant and diverse endophytic actinobacteria associated with medicinal plant Maytenus austroyunnanensis in Xishuangbanna tropical rainforest revealed by culture-dependent and culture-independent methods,ENVIRONMENTAL MICROBIOLOGY REPORTS,2012,4(5):522-531
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