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Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides
Ou-Yang, ZL; Wang, PR; Huang, XH; Cai, J; Huang, YH; Wei, SN; Ji, HS; Wei, JG; Zhou, YC; Qin, QW; qinqw@scsio.ac.cn
2012
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume38Issue:2Pages:254-261
AbstractVaccination is one of the best methods against viral diseases. In this study, experimental inactivated Singapore grouper iridovirus (SGIV) vaccines were prepared, and immunogenicity and protection against virus infection of the vaccines were investigated in orange-spotted grouper, Epinephelus coioides. Two kinds of vaccines, including,beta-propiolactone (BPL) inactivated virus at 4 degrees C for 12 h and formalin inactivated virus at 4 degrees C for 12 d, was highly protective against the challenge at 30-day post-vaccination and produced relative percent of survival rates of 91.7% and 100%, respectively. These effective vaccinations induced potent innate immune responses mediated by pro-inflammatory cytokines and type! interferon (IFN)-stimulated genes (ISGs). It is noteworthy that ISGs, such as Mx and ISG15, were up-regulated only in the effective vaccine groups, which suggested that type 1 IFN system may be the functional basis of early anti-viral immunity. Moreover, effective vaccination also significantly up-regulated of the expression of MHC class I gene and produced substantial amount of specific serum antibody at 4 weeks post-vaccination. Taken together, our results clearly demonstrated that effective vaccination in grouper induced an early, nonspecific antiviral immunity, and later, a specific immune response involving both humoral and cell-mediated immunity. (C) 2012 Elsevier Ltd. All rights reserved.
Department[Ou-yang, Zhengliang; Huang, Xiaohong; Cai, Jia; Huang, Youhua; Wei, Shina; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Peiran] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; [Ji, Huasong; Zhou, Yongcan] Hainan Univ, Coll Marine Sci, Key Lab Trop Biol Resources, Haikou 570228, Peoples R China
KeywordSingapore Grouper Iridovirus (Sgiv) Inactivated Vaccine Formalin Beta-propiolactone Elisa Immune-related Genes
Subject AreaImmunology ; Zoology
Language英语
Funding ProjectLMB
WOS IDWOS:000309312700007
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9792
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
Ou-Yang, ZL,Wang, PR,Huang, XH,et al. Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(2):254-261.
APA Ou-Yang, ZL.,Wang, PR.,Huang, XH.,Cai, J.,Huang, YH.,...&qinqw@scsio.ac.cn.(2012).Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,38(2),254-261.
MLA Ou-Yang, ZL,et al."Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 38.2(2012):254-261.
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