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学科主题: Biochemistry & Molecular Biology
题名: A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS
作者: Wang, XX ; Lord, DM ; Cheng, HY ; Osbourne, DO ; Hong, SH ; Sanchez-Torres, V ; Quiroga, C ; Zheng, K ; Herrmann, T ; Peti, W ; Benedik, MJ ; Page, R ; Wood, TK
通讯作者: Rebecca_Page@brown.edu ; TWood@engr.psu.edu
刊名: NATURE CHEMICAL BIOLOGY
发表日期: 2012
卷: 8, 期:10, 页:855-861
部门归属: [Zheng, Kevin; Page, Rebecca] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA; [Wang, Xiaoxue] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China; [Wang, Xiaoxue; Hong, Seok Hoon; Sanchez-Torres, Viviana; Wood, Thomas K.] Texas A&M Univ, Dept Chem Engn, College Stn, TX 77843 USA; [Lord, Dana M.; Peti, Wolfgang] Brown Univ, Dept Mol Pharmacol Physiol & Biotechnol, Providence, RI 02912 USA; [Cheng, Hsin-Yao; Osbourne, Devon O.; Quiroga, Cecilia; Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA; [Herrmann, Torsten] Univ Lyon, Ctr Europeen Resonance Magnet Nucl Tres Champ, CNRS, Ecole Normale Super Lyon, Lyon, France; [Benedik, Michael J.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA; [Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
项目归属: LMB
摘要: Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA.
语种: 英语
WOS记录号: WOS:000309056200011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9796
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wang, XX; Lord, DM; Cheng, HY; Osbourne, DO; Hong, SH; Sanchez-Torres, V; Quiroga, C; Zheng, K; Herrmann, T; Peti, W; Benedik, MJ; Page, R; Wood, TK.A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS,NATURE CHEMICAL BIOLOGY,2012,8(10):855-861
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