A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS
Wang, XX; Lord, DM; Cheng, HY; Osbourne, DO; Hong, SH; Sanchez-Torres, V; Quiroga, C; Zheng, K; Herrmann, T; Peti, W; Benedik, MJ; Page, R; Wood, TK; Rebecca_Page@brown.edu; TWood@engr.psu.edu
2012
发表期刊NATURE CHEMICAL BIOLOGY
ISSN1552-4450
卷号8期号:10页码:855-861
摘要Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA.
部门归属[Zheng, Kevin; Page, Rebecca] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA; [Wang, Xiaoxue] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China; [Wang, Xiaoxue; Hong, Seok Hoon; Sanchez-Torres, Viviana; Wood, Thomas K.] Texas A&M Univ, Dept Chem Engn, College Stn, TX 77843 USA; [Lord, Dana M.; Peti, Wolfgang] Brown Univ, Dept Mol Pharmacol Physiol & Biotechnol, Providence, RI 02912 USA; [Cheng, Hsin-Yao; Osbourne, Devon O.; Quiroga, Cecilia; Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA; [Herrmann, Torsten] Univ Lyon, Ctr Europeen Resonance Magnet Nucl Tres Champ, CNRS, Ecole Normale Super Lyon, Lyon, France; [Benedik, Michael J.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA; [Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
学科领域Biochemistry & Molecular Biology
语种英语
资助项目LMB
WOS记录号WOS:000309056200011
引用统计
被引频次:137[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9796
专题中科院海洋生物资源可持续利用重点实验室
通讯作者Rebecca_Page@brown.edu; TWood@engr.psu.edu
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Wang, XX,Lord, DM,Cheng, HY,et al. A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS[J]. NATURE CHEMICAL BIOLOGY,2012,8(10):855-861.
APA Wang, XX.,Lord, DM.,Cheng, HY.,Osbourne, DO.,Hong, SH.,...&TWood@engr.psu.edu.(2012).A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.NATURE CHEMICAL BIOLOGY,8(10),855-861.
MLA Wang, XX,et al."A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS".NATURE CHEMICAL BIOLOGY 8.10(2012):855-861.
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