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学科主题: Food Science & Technology
题名: Identification of 11 sea cucumber species by species-specific PCR method
作者: Wen, J ; Hu, CQ ; Zhang, LP ; Fan, SG
通讯作者: jw82123@yahoo.com.cn
关键词: Sea cucumber ; Species-specific PCR ; Identification ; 16S rRNA gene
刊名: FOOD CONTROL
发表日期: 2012
卷: 27, 期:2, 页:380-384
部门归属: [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Fan, Sigang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Fan, Sigang] Chinese Acad Sci, Chinese Acad Sci LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing] Zhanjiang Normal Univ, Dept Biol, Zhanjiang 524048, Peoples R China
项目归属: LMB
摘要: A species-specific PCR (polymerase chain reaction) method was developed to identify the 11 sea cucumber species, even in highly processed food samples such as frozen or dried sea cucumbers (beche-de-mer). Universal primers were used for the amplification of 16S rRNA gene in each species. The alignment of the obtained sequences was the basis for the design of 11 species-specific reverse primers located in the 16S rRNA gene region. The developed species-specific PCR method was successfully applied to authenticate species of commercial sea cucumber products. Therefore, this method really provides a simple, fast and reliable protocol for the accurate identification of sea cucumber species, and also can be very useful for correct labeling of commercial products, traceability, and protection of the consumer's rights. (c) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
WOS记录号: WOS:000305878100017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9798
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Wen, J; Hu, CQ; Zhang, LP; Fan, SG.Identification of 11 sea cucumber species by species-specific PCR method,FOOD CONTROL,2012,27(2):380-384
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