Identification of 11 sea cucumber species by species-specific PCR method
Wen, J; Hu, CQ; Zhang, LP; Fan, SG; jw82123@yahoo.com.cn
2012
发表期刊FOOD CONTROL
ISSN0956-7135
卷号27期号:2页码:380-384
摘要A species-specific PCR (polymerase chain reaction) method was developed to identify the 11 sea cucumber species, even in highly processed food samples such as frozen or dried sea cucumbers (beche-de-mer). Universal primers were used for the amplification of 16S rRNA gene in each species. The alignment of the obtained sequences was the basis for the design of 11 species-specific reverse primers located in the 16S rRNA gene region. The developed species-specific PCR method was successfully applied to authenticate species of commercial sea cucumber products. Therefore, this method really provides a simple, fast and reliable protocol for the accurate identification of sea cucumber species, and also can be very useful for correct labeling of commercial products, traceability, and protection of the consumer's rights. (c) 2012 Elsevier Ltd. All rights reserved.
部门归属[Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Fan, Sigang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Fan, Sigang] Chinese Acad Sci, Chinese Acad Sci LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing] Zhanjiang Normal Univ, Dept Biol, Zhanjiang 524048, Peoples R China
关键词Sea Cucumber Species-specific Pcr Identification 16s Rrna Gene
学科领域Food Science & Technology
语种英语
资助项目LMB
WOS记录号WOS:000305878100017
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9798
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jw82123@yahoo.com.cn
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GB/T 7714
Wen, J,Hu, CQ,Zhang, LP,et al. Identification of 11 sea cucumber species by species-specific PCR method[J]. FOOD CONTROL,2012,27(2):380-384.
APA Wen, J,Hu, CQ,Zhang, LP,Fan, SG,&jw82123@yahoo.com.cn.(2012).Identification of 11 sea cucumber species by species-specific PCR method.FOOD CONTROL,27(2),380-384.
MLA Wen, J,et al."Identification of 11 sea cucumber species by species-specific PCR method".FOOD CONTROL 27.2(2012):380-384.
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