SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Identification of 11 sea cucumber species by species-specific PCR method
Wen, J; Hu, CQ; Zhang, LP; Fan, SG; jw82123@yahoo.com.cn
2012
Source PublicationFOOD CONTROL
ISSN0956-7135
Volume27Issue:2Pages:380-384
AbstractA species-specific PCR (polymerase chain reaction) method was developed to identify the 11 sea cucumber species, even in highly processed food samples such as frozen or dried sea cucumbers (beche-de-mer). Universal primers were used for the amplification of 16S rRNA gene in each species. The alignment of the obtained sequences was the basis for the design of 11 species-specific reverse primers located in the 16S rRNA gene region. The developed species-specific PCR method was successfully applied to authenticate species of commercial sea cucumber products. Therefore, this method really provides a simple, fast and reliable protocol for the accurate identification of sea cucumber species, and also can be very useful for correct labeling of commercial products, traceability, and protection of the consumer's rights. (c) 2012 Elsevier Ltd. All rights reserved.
Department[Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Fan, Sigang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing; Hu, Chaoqun; Zhang, Lvping; Fan, Sigang] Chinese Acad Sci, Chinese Acad Sci LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wen, Jing] Zhanjiang Normal Univ, Dept Biol, Zhanjiang 524048, Peoples R China
KeywordSea Cucumber Species-specific Pcr Identification 16s Rrna Gene
Subject AreaFood Science & Technology
Language英语
Funding ProjectLMB
WOS IDWOS:000305878100017
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9798
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorjw82123@yahoo.com.cn
Recommended Citation
GB/T 7714
Wen, J,Hu, CQ,Zhang, LP,et al. Identification of 11 sea cucumber species by species-specific PCR method[J]. FOOD CONTROL,2012,27(2):380-384.
APA Wen, J,Hu, CQ,Zhang, LP,Fan, SG,&jw82123@yahoo.com.cn.(2012).Identification of 11 sea cucumber species by species-specific PCR method.FOOD CONTROL,27(2),380-384.
MLA Wen, J,et al."Identification of 11 sea cucumber species by species-specific PCR method".FOOD CONTROL 27.2(2012):380-384.
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