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学科主题: Immunology; Veterinary Sciences
题名: Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination
作者: Ou-Yang, Z ; Wang, PR ; Huang, YH ; Huang, XH ; Wan, QJ ; Zhou, S ; Wei, JG ; Zhou, YC ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: Singapore grouper iridovirus (SGIV) ; Bioinformatics ; DNA vaccine ; Grouper
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2012
卷: 149, 期:41276, 页:38-45
部门归属: [Ou-yang, Zhengliang; Huang, Youhua; Huang, Xiaohong; Zhou, Sheng; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Peiran; Wan, Qingjiao] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; [Zhou, Yongcan] Hainan Univ, Coll Marine Sci, Key Lab Trop Biol Resources, Haikou 570228, Peoples R China
项目归属: LMB
摘要: In this study, we described a rapid and efficient method which integrated the bioinformatic prediction and DNA vaccine technology to identify vaccine candidates against Singapore grouper iridovirus (SGIV). The 162 previously defined open reading frames (ORFs) of SGIV were subjected to extensive sequence similarity searches, as well as motif, cellular location, and domain prediction. Based on our analysis, 13 genes were chosen and cloned into the eukaryotic expression vector pcDNA 3.1. In vitro and in vivo expression of these DNA vaccine constructs was examined in Epinephelus akaara spleen cells (EAGS) and immunized fish by Western blot and RT-PCR analysis, respectively. Three weeks after the second booster, immunized fish were challenged with SGIV and the level of protection and survival was assessed. Fish vaccinated with plasmid DNA encoding viral ORF072, ORF039 and ORF036 (designated as pcDNA-72, pcDNA-39 and pcDNA-36, respectively) exhibited 66.7%, 66.7% and 58.3% relative percent survival rates, respectively, in comparison with the control fish. These three DNA vaccines induced innate immune responses, raising significantly high level of Mx expression relative to the fish vaccinated with the empty plasmid at 3 days post-vaccination. Furthermore, recombinant protein from ORF072 was also used to immunize another set of fish and similar protective effect was obtained. Taken together, our results validated the applicability of bioinformatics in genome mining, resulting in the identification of three protective antigens. The promising results obtained in the present study have prompted further testing to improve the immunogenicity of these potential DNA vaccines. (C) 2012 Elsevier B.V. All rights reserved.
语种: 英语
WOS记录号: WOS:000308831300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9802
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Ou-Yang, Z; Wang, PR; Huang, YH; Huang, XH; Wan, QJ; Zhou, S; Wei, JG; Zhou, YC; Qin, QW.Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2012,149(41276):38-45
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