Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination
Ou-Yang, Z; Wang, PR; Huang, YH; Huang, XH; Wan, QJ; Zhou, S; Wei, JG; Zhou, YC; Qin, QW; qinqw@scsio.ac.cn
2012
发表期刊VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
卷号149期号:41276页码:38-45
摘要In this study, we described a rapid and efficient method which integrated the bioinformatic prediction and DNA vaccine technology to identify vaccine candidates against Singapore grouper iridovirus (SGIV). The 162 previously defined open reading frames (ORFs) of SGIV were subjected to extensive sequence similarity searches, as well as motif, cellular location, and domain prediction. Based on our analysis, 13 genes were chosen and cloned into the eukaryotic expression vector pcDNA 3.1. In vitro and in vivo expression of these DNA vaccine constructs was examined in Epinephelus akaara spleen cells (EAGS) and immunized fish by Western blot and RT-PCR analysis, respectively. Three weeks after the second booster, immunized fish were challenged with SGIV and the level of protection and survival was assessed. Fish vaccinated with plasmid DNA encoding viral ORF072, ORF039 and ORF036 (designated as pcDNA-72, pcDNA-39 and pcDNA-36, respectively) exhibited 66.7%, 66.7% and 58.3% relative percent survival rates, respectively, in comparison with the control fish. These three DNA vaccines induced innate immune responses, raising significantly high level of Mx expression relative to the fish vaccinated with the empty plasmid at 3 days post-vaccination. Furthermore, recombinant protein from ORF072 was also used to immunize another set of fish and similar protective effect was obtained. Taken together, our results validated the applicability of bioinformatics in genome mining, resulting in the identification of three protective antigens. The promising results obtained in the present study have prompted further testing to improve the immunogenicity of these potential DNA vaccines. (C) 2012 Elsevier B.V. All rights reserved.
部门归属[Ou-yang, Zhengliang; Huang, Youhua; Huang, Xiaohong; Zhou, Sheng; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Peiran; Wan, Qingjiao] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; [Zhou, Yongcan] Hainan Univ, Coll Marine Sci, Key Lab Trop Biol Resources, Haikou 570228, Peoples R China
关键词Singapore Grouper Iridovirus (Sgiv) Bioinformatics Dna Vaccine Grouper
学科领域Immunology ; Veterinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000308831300006
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9802
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Ou-Yang, Z,Wang, PR,Huang, YH,et al. Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2012,149(41276):38-45.
APA Ou-Yang, Z.,Wang, PR.,Huang, YH.,Huang, XH.,Wan, QJ.,...&qinqw@scsio.ac.cn.(2012).Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,149(41276),38-45.
MLA Ou-Yang, Z,et al."Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 149.41276(2012):38-45.
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