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Prevalent Ciliate Symbiosis on Copepods: High Genetic Diversity and Wide Distribution Detected Using Small Subunit Ribosomal RNA Gene
Guo, ZL; Liu, S; Hu, SM; Li, T; Huang, YS; Liu, GX; Zhang, H; Lin, SJ; huan.zhang@uconn.edu; senjie.lin@uconn.edu
2012
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:9Pages:e44847
AbstractToward understanding the genetic diversity and distribution of copepod-associated symbiotic ciliates and the evolutionary relationships with their hosts in the marine environment, we developed a small subunit ribosomal RNA gene (18S rDNA)based molecular method and investigated the genetic diversity and genotype distribution of the symbiotic ciliates on copepods. Of the 10 copepod species representing six families collected from six locations of Pacific and Atlantic Oceans, 9 were found to harbor ciliate symbionts. Phylogenetic analysis of the 391 ciliate 18S rDNA sequences obtained revealed seven groups (ribogroups), six (containing 99% of all the sequences) belonging to subclass Apostomatida, the other clustered with peritrich ciliate Vorticella gracilis. Among the Apostomatida groups, Group III were essentially identical to Vampyrophrya pelagica, and the other five groups represented the undocumented ciliates that were close to Vampyrophrya/Gymnodinioides/Hyalophysa. Group VI ciliates were found in all copepod species but one (Calanus sinicus), and were most abundant among all ciliate sequences obtained, indicating that they are the dominant symbiotic ciliates universally associated with copepods. In contrast, some ciliate sequences were found only in some of the copepods examined, suggesting the host selectivity and geographic differentiation of ciliates, which requires further verification by more extensive sampling. Our results reveal the wide occurrence and high genetic diversity of symbiotic ciliates on marine copepods and highlight the need to systematically investigate the host- and geography-based genetic differentiation and ecological roles of these ciliates globally.
Department[Guo, Zhiling; Liu, Sheng; Hu, Simin; Li, Tao] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Guo, Zhiling; Zhang, Huan; Lin, Senjie] Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA; [Lin, Senjie] Xiamen Univ, Marine Biodivers & Global Change Lab, Xiamen, Fujian, Peoples R China; [Huang, Yousong; Liu, Guangxing; Zhang, Huan] Ocean Univ China, Dept Environm Sci, Qingdao, Shandong, Peoples R China
Subject AreaMultidisciplinary Sciences
Language英语
Funding ProjectLMB
WOS IDWOS:000308860100026
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9804
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhuan.zhang@uconn.edu; senjie.lin@uconn.edu
Recommended Citation
GB/T 7714
Guo, ZL,Liu, S,Hu, SM,et al. Prevalent Ciliate Symbiosis on Copepods: High Genetic Diversity and Wide Distribution Detected Using Small Subunit Ribosomal RNA Gene[J]. PLOS ONE,2012,7(9):e44847.
APA Guo, ZL.,Liu, S.,Hu, SM.,Li, T.,Huang, YS.,...&senjie.lin@uconn.edu.(2012).Prevalent Ciliate Symbiosis on Copepods: High Genetic Diversity and Wide Distribution Detected Using Small Subunit Ribosomal RNA Gene.PLOS ONE,7(9),e44847.
MLA Guo, ZL,et al."Prevalent Ciliate Symbiosis on Copepods: High Genetic Diversity and Wide Distribution Detected Using Small Subunit Ribosomal RNA Gene".PLOS ONE 7.9(2012):e44847.
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