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学科主题: Biochemistry & Molecular Biology; Genetics & Heredity
题名: Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization
作者: Wang, YH ; Fu, DK ; Luo, P ; He, XC
通讯作者: yhwang@scsio.ac.cn
关键词: Immune-relevant gene ; Pinctada martensii ; Suppression Subtractive Hybridization (SSH) ; Vibrio alginolyticus
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
发表日期: 2012
卷: 7, 期:3, 页:243-247
部门归属: [Wang, Yanhong] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Key Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Guangdong Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: One hemolymph subtracted cDNA library of pearl oyster (Pinctada martensii, Dunker 1837) was constructed using the suppression subtractive hybridization (SSH) in response to Vibrio alginolyticus. A total of 1089 clones were sequenced. All the consensuses were recognized based on the BLAST searches in NCBI, and revealed that 376 (58%) of them had no significant matches to reported sequences in the database. 267 ESTs were in significant matches after homologous sequence searches. Hypothesized genes inferred from EST sequences were categorized into six groups according to their putative biological functions: replication, transcription and translation: cellular processes; responded to stimuli; metabolism and biosynthesis: signal transduction genes; "other" category. The five genes, pearlin gene promoter PGPPm, serine/threonine kinase STKPm, limbic system-associated membrane protein LSAMPPm, nacrein gene intron 6 NGIPm6 and ferritin-like protein FLPPm, were analyzed using real-time PCR. All these genes were significantly expressed after V. alginolyticus challenge. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
语种: 英语
WOS记录号: WOS:000308115900002
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9806
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Wang, YH; Fu, DK; Luo, P; He, XC.Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2012,7(3):243-247
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