Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization
Wang, YH; Fu, DK; Luo, P; He, XC; yhwang@scsio.ac.cn
2012
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN1744-117X
卷号7期号:3页码:243-247
摘要One hemolymph subtracted cDNA library of pearl oyster (Pinctada martensii, Dunker 1837) was constructed using the suppression subtractive hybridization (SSH) in response to Vibrio alginolyticus. A total of 1089 clones were sequenced. All the consensuses were recognized based on the BLAST searches in NCBI, and revealed that 376 (58%) of them had no significant matches to reported sequences in the database. 267 ESTs were in significant matches after homologous sequence searches. Hypothesized genes inferred from EST sequences were categorized into six groups according to their putative biological functions: replication, transcription and translation: cellular processes; responded to stimuli; metabolism and biosynthesis: signal transduction genes; "other" category. The five genes, pearlin gene promoter PGPPm, serine/threonine kinase STKPm, limbic system-associated membrane protein LSAMPPm, nacrein gene intron 6 NGIPm6 and ferritin-like protein FLPPm, were analyzed using real-time PCR. All these genes were significantly expressed after V. alginolyticus challenge. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
部门归属[Wang, Yanhong] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Key Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Guangdong Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
关键词Immune-relevant Gene Pinctada Martensii Suppression Subtractive Hybridization (Ssh) Vibrio Alginolyticus
学科领域Biochemistry & Molecular Biology ; Genetics & Heredity
语种英语
资助项目LMB
WOS记录号WOS:000308115900002
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9806
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yhwang@scsio.ac.cn
推荐引用方式
GB/T 7714
Wang, YH,Fu, DK,Luo, P,et al. Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2012,7(3):243-247.
APA Wang, YH,Fu, DK,Luo, P,He, XC,&yhwang@scsio.ac.cn.(2012).Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,7(3),243-247.
MLA Wang, YH,et al."Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 7.3(2012):243-247.
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