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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Molecular cloning and characterization of class I NF-kappa B transcription factor from pearl oyster (Pinctada fucata)
作者: Huang, XD ; Liu, WG ; Guan, YY ; Shi, Y ; Wang, Q ; Zhao, M ; Wu, SZ ; He, MX
通讯作者: hmx@scsio.ac.cn
关键词: Pearl oyster ; Class I NIF kappa B transcription factor ; Gene cloning ; Immunity response
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2012
卷: 33, 期:3, 页:659-666
部门归属: [Huang, Xian-De; Liu, Wen-Guang; Guan, Yun-Yan; Shi, Yu; Wang, Qi; Zhao, Mi; Wu, Shan-Zeng; He, Mao-Xian] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: NF-kappa B transcription factors play central roles in many important physiological and pathological processes including innate immune responses. Here we report the cloning of an NF-kappa B transcription factor. PfRelish from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfRelish full-length cDNA is 3916 bp with an open reading frame of 3558 bp encoding a putative protein of 1186 amino acids. The deduced PfRelish contains a N-terminal RHD, a nucleus localization signal, an I kappa B-like domain with six ankyrin repeats and a death domain at the C-terminus, which is similar to class I NF-kappa B transcription factors. Comparison and phylogenetic analysis revealed that class I NF-kappa Bs in mollusks including PfRelish might have most distant relationship to the arthropod Relish. Further expression analysis showed that PfRelish was apparently upregulated after Vibrio alginolyticus injection, which suggested that PfRelish was involved in the immune response to V. alginolyticus. (C) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
WOS记录号: WOS:000308057700025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9822
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Huang, XD; Liu, WG; Guan, YY; Shi, Y; Wang, Q; Zhao, M; Wu, SZ; He, MX.Molecular cloning and characterization of class I NF-kappa B transcription factor from pearl oyster (Pinctada fucata),FISH & SHELLFISH IMMUNOLOGY,2012,33(3):659-666
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