Isolation and characterization of a thioredoxin domain-containing protein 12 from orange-spotted grouper, Epinephelus coioides
Wei, JG; Ji, HS; Guo, ML; Qin, QW; qinqw@scsio.ac.cn
2012
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号33期号:3页码:667-673
摘要Thioredoxin domain-containing protein 12 (Txndc12) belongs to the thioredoxin superfamily, and has roles in redox regulation, defense against oxidative stress, refolding of disulfide-containing proteins, and regulation of transcription factors. In this study, a thioredoxin domain-containing protein 12 was cloned from the marine fish grouper. Epinephelus coioides by RACE PCR, named as Ec-Txndc12. The Ec-Txndc12 encodes 173 amino acid residues with signal peptide in its N-terminal and a thioredoxin (Trx) domain that is homologous with some genes in Mus musculus, Xenopus laveis, etc. Ec-Txndc12 mRNA is predominately expressed in liver, brain and muscle. The expression of Ec-Txndc12 was up-regulated in the liver of grouper challenged with SGIV. In order to elucidate its biological functions. Ec-Txndc12 was recombined and expressed in Escherichia coli BL21 (DE3). The rEc-Txndc12 fusion protein was demonstrated to possess the antioxidant activity. The grouper spleen (GS) cells were treated with a high concentration of rEc-Txndc12 (30 mu g/ml), which significantly enhanced cells viability under oxidative damage caused by viral infection. These results together indicated that Ec-Txndc12 could function as an important antioxidant in a physiological context, and might be involved in the responses to viral challenge. (C) 2012 Elsevier Ltd. All rights reserved.
部门归属[Wei, Jingguang; Guo, Minglan; Qin, Qiwei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ji, Huasong] Hainan Univ, State Key Lab Breeding Base Sustainable Exploitat, Coll Marine Sci, Haikou 570228, Peoples R China
关键词Epinephelus Coioides Singapore Grouper Iridovirus (Sgiv) Thioredoxin Domain-containing Protein 12 (Txndc12) Antioxidant Activity
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000308057700026
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9824
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Wei, JG,Ji, HS,Guo, ML,et al. Isolation and characterization of a thioredoxin domain-containing protein 12 from orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):667-673.
APA Wei, JG,Ji, HS,Guo, ML,Qin, QW,&qinqw@scsio.ac.cn.(2012).Isolation and characterization of a thioredoxin domain-containing protein 12 from orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,33(3),667-673.
MLA Wei, JG,et al."Isolation and characterization of a thioredoxin domain-containing protein 12 from orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):667-673.
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