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学科主题: Immunology; Zoology
题名: Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)
作者: Wei, JG ; Guo, ML ; Ji, HS ; Yan, Y ; Ouyang, ZL ; Huang, XH ; Hang, YH ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: Thioredoxin ; Epinephelus coioides ; SGIV ; Intracellular localization ; Antioxidant capacity
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2012
卷: 38, 期:1, 页:108-116
部门归属: [Wei, Jingguang; Guo, Minglan; Yan, Yang; Ouyang, Zhengliang; Huang, Xiaohong; Hang, Youhua; Qin, Qiwei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
项目归属: LMB
摘要: Thioredoxins (TRXs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. In this study, a thioredoxin gene was cloned from orange-spotted grouper, Epinephelus coioides (designated as Ec-TRX). The full-length cDNA of Ec-TRX was comprised of 767 bp with a 327 bp open reading frame that encodes a putative protein of 108 amino acids. Quantitative real-time PCR analysis revealed that the Ec-TRX mRNA was distributed abundantly in grouper, E. coioides skin and liver, and the expression in liver was up-regulated after viral challenge with Singapore grouper iridovirus (SGIV). Recombinant Ec-TRX (rEc-TRX) was expressed in Escherichia coli BL21 (DE3) and purified for mouse anti-Ec-TRX serum preparation. The rEc-TRX fusion protein was demonstrated to possess the expected redox activity in enzymatic analysis, and scavenge free radicals and protect supercoiled DNA from oxidative damage induced by a metal-ion catalyzed oxidation reaction. Subcellular localization revealed that Ec-TRX was distributed in both cytoplasm and nucleus. Overexpression of Ec-TRX in grouper spleen (GS) cells could promote the growth of GS cells and inhibit the replication of SGIV. These results suggest that Ec-TRX could function as an important antioxidant in a physiological context, and perhaps is involved in the responses to viral challenge. (C) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
WOS记录号: WOS:000308271100012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9826
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wei, JG; Guo, ML; Ji, HS; Yan, Y; Ouyang, ZL; Huang, XH; Hang, YH; Qin, QW.Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides),DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(1):108-116
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文件名: Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper.pdf
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