Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)
Wei, JG; Guo, ML; Ji, HS; Yan, Y; Ouyang, ZL; Huang, XH; Hang, YH; Qin, QW; qinqw@scsio.ac.cn
2012
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
卷号38期号:1页码:108-116
摘要Thioredoxins (TRXs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. In this study, a thioredoxin gene was cloned from orange-spotted grouper, Epinephelus coioides (designated as Ec-TRX). The full-length cDNA of Ec-TRX was comprised of 767 bp with a 327 bp open reading frame that encodes a putative protein of 108 amino acids. Quantitative real-time PCR analysis revealed that the Ec-TRX mRNA was distributed abundantly in grouper, E. coioides skin and liver, and the expression in liver was up-regulated after viral challenge with Singapore grouper iridovirus (SGIV). Recombinant Ec-TRX (rEc-TRX) was expressed in Escherichia coli BL21 (DE3) and purified for mouse anti-Ec-TRX serum preparation. The rEc-TRX fusion protein was demonstrated to possess the expected redox activity in enzymatic analysis, and scavenge free radicals and protect supercoiled DNA from oxidative damage induced by a metal-ion catalyzed oxidation reaction. Subcellular localization revealed that Ec-TRX was distributed in both cytoplasm and nucleus. Overexpression of Ec-TRX in grouper spleen (GS) cells could promote the growth of GS cells and inhibit the replication of SGIV. These results suggest that Ec-TRX could function as an important antioxidant in a physiological context, and perhaps is involved in the responses to viral challenge. (C) 2012 Elsevier Ltd. All rights reserved.
部门归属[Wei, Jingguang; Guo, Minglan; Yan, Yang; Ouyang, Zhengliang; Huang, Xiaohong; Hang, Youhua; Qin, Qiwei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
关键词Thioredoxin Epinephelus Coioides Sgiv Intracellular Localization Antioxidant Capacity
学科领域Immunology ; Zoology
语种英语
资助项目LMB
WOS记录号WOS:000308271100012
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9826
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Wei, JG,Guo, ML,Ji, HS,et al. Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(1):108-116.
APA Wei, JG.,Guo, ML.,Ji, HS.,Yan, Y.,Ouyang, ZL.,...&qinqw@scsio.ac.cn.(2012).Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,38(1),108-116.
MLA Wei, JG,et al."Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 38.1(2012):108-116.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Cloning, characteriz(1284KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。