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学科主题: Multidisciplinary Sciences
题名: Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides
作者: Yan, Y ; Cui, HC ; Wei, JG ; Huang, YH ; Huang, XH ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: myeloid differentiation factor 88 (MyD88) ; Epinephelus coioides ; expression analysis ; NF-kappa B activation ; immune response ; SGIV
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2012
卷: 57, 期:25, 页:3277-3287
部门归属: [Yan Yang; Wei JingGuang; Huang YouHua; Huang XiaoHong; Qin QiWei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Cui HuaChun] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
摘要: Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein involved in Toll-like receptors and in interleukin-1 receptor-induced nuclear factor-kappaB (NF-kappa B) activation. In this study, a new MyD88 gene (designated as Og-MyD88) was cloned from orange-spotted grouper, Epinephelus coioides, based on the expressed sequence tag (EST) obtained following Roche 454 GS-FLX (TM) sequencing. The full-length Og-MyD88 cDNA is composed of 1682 bp and encodes a deduced polypeptide of 289 amino acids with 86% homology to MyD88 of Siniperca chuatsi. The deduced amino acid sequence of Og-MyD88 contains a typical death domain at the amino terminus and a conserved Toll/IL-1R (TIR) domain at the carboxyl terminus, as well as three highly conserved motifs (Box1, Box2 and Box3) within the C-terminal TIR domain. In healthy fish, Og-MyD88 was found to be strongly expressed in immune-related tissues, including the spleen, head kidney, kidney, liver, skin and intestine, with lower expression in heart, stomach, brain and muscle. Transcripts of Og-MyD88 were found to be markedly up-regulated in fish spleen after challenge with Singapore grouper iridovirus (SGIV), a highly lethal viral pathogen to grouper fish. Furthermore, the full length Og-MyD88 and its N-terminal death domain were capable of inducing NF-kappa B activity in HEK-293 cells. Overexpressed Og-MyD88 showed the ability to inhibit replication of SGIV in grouper spleen (GS) cells. These results suggest that Og-MyD88 is involved in the grouper immune response to invasion of viral pathogens and may share similar functions to those observed in higher vertebrates.
语种: 英语
WOS记录号: WOS:000307750500005
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9834
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Yan, Y; Cui, HC; Wei, JG; Huang, YH; Huang, XH; Qin, QW.Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides,CHINESE SCIENCE BULLETIN,2012,57(25):3277-3287
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