Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides
Yan, Y; Cui, HC; Wei, JG; Huang, YH; Huang, XH; Qin, QW; qinqw@scsio.ac.cn
2012
发表期刊CHINESE SCIENCE BULLETIN
ISSN1001-6538
卷号57期号:25页码:3277-3287
摘要Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein involved in Toll-like receptors and in interleukin-1 receptor-induced nuclear factor-kappaB (NF-kappa B) activation. In this study, a new MyD88 gene (designated as Og-MyD88) was cloned from orange-spotted grouper, Epinephelus coioides, based on the expressed sequence tag (EST) obtained following Roche 454 GS-FLX (TM) sequencing. The full-length Og-MyD88 cDNA is composed of 1682 bp and encodes a deduced polypeptide of 289 amino acids with 86% homology to MyD88 of Siniperca chuatsi. The deduced amino acid sequence of Og-MyD88 contains a typical death domain at the amino terminus and a conserved Toll/IL-1R (TIR) domain at the carboxyl terminus, as well as three highly conserved motifs (Box1, Box2 and Box3) within the C-terminal TIR domain. In healthy fish, Og-MyD88 was found to be strongly expressed in immune-related tissues, including the spleen, head kidney, kidney, liver, skin and intestine, with lower expression in heart, stomach, brain and muscle. Transcripts of Og-MyD88 were found to be markedly up-regulated in fish spleen after challenge with Singapore grouper iridovirus (SGIV), a highly lethal viral pathogen to grouper fish. Furthermore, the full length Og-MyD88 and its N-terminal death domain were capable of inducing NF-kappa B activity in HEK-293 cells. Overexpressed Og-MyD88 showed the ability to inhibit replication of SGIV in grouper spleen (GS) cells. These results suggest that Og-MyD88 is involved in the grouper immune response to invasion of viral pathogens and may share similar functions to those observed in higher vertebrates.
部门归属[Yan Yang; Wei JingGuang; Huang YouHua; Huang XiaoHong; Qin QiWei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Cui HuaChun] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
关键词Myeloid Differentiation Factor 88 (Myd88) Epinephelus Coioides Expression Analysis Nf-kappa b Activation Immune Response Sgiv
学科领域Multidisciplinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000307750500005
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9834
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Yan, Y,Cui, HC,Wei, JG,et al. Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides[J]. CHINESE SCIENCE BULLETIN,2012,57(25):3277-3287.
APA Yan, Y.,Cui, HC.,Wei, JG.,Huang, YH.,Huang, XH.,...&qinqw@scsio.ac.cn.(2012).Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides.CHINESE SCIENCE BULLETIN,57(25),3277-3287.
MLA Yan, Y,et al."Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides".CHINESE SCIENCE BULLETIN 57.25(2012):3277-3287.
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