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Molecular identification of an androgen receptor and its changes in mRNA levels during 17 alpha-methyltestosterone-induced sex reversal in the orange-spotted grouper Epinephelus coioides
Shi, Y; Liu, XC; Zhang, HF; Zhang, Y; Lu, DQ; Lin, HR; lsslhr@mail.sysu.edu.cn
2012
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume163Issue:1Pages:43-50
AbstractAndrogens play a crucial role in sex differentiation, sexual maturation, and spermatogenesis in vertebrates. The action of androgens is mediated via androgen receptors (ARs). The present study reports the cloning of the cDNA sequence of the or in the orange-spotted grouper, with high expression in testis and relatively low in subdivision of brain areas. The cDNA sequence of or was 2358 bp, encoding a protein of 759 amino acids (aa). Phylogenetic analysis showed that the at cDNA sequence was closely related to that of threespot wrasse (Halichoeres trimaculatus) and medaka (Oryzias latipes) ar beta. As deduced from the phylogenetic tree and the high amino acid identity with the AR beta, subtype of other teleosts, grouper or seems to be more closely related to the beta than the alpha subtype cloned to date. In the first week after 17 alpha-methyltestosterone (MT) implantation, the transcript levels of or in the hypothalamus declined significantly, and consistently stayed at low level expression to the second week, but increased back to the control levels in the third and fourth week. In the gonad, the mRNA expression of or was not changed in the first week compared with the control, but increased significantly in the second week, consistently reached the highest level in the third week, dropped slightly but still higher than that of the control in the fourth week. The expression pattern of or in hypothalamus and gonad during MT-induced sex reversal suggests the involvement of or in regulating this process in the orange-spotted grouper. The present study provides the data of the changes in the mRNA levels of or during MT-induced sex reversal in detail to help understand the complicated signals under sex reversal. (C) 2012 Elsevier Inc. All rights reserved.
Department[Shi, Yu; Liu, Xiaochun; Zhang, Yong; Lu, Danqi; Lin, Haoran] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China; [Shi, Yu; Liu, Xiaochun; Zhang, Yong; Lu, Danqi; Lin, Haoran] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; [Shi, Yu] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Haifa] Guangdong Daya Bay Fishery Dev Ctr, Huizhou 516081, Peoples R China; [Lin, Haoran] Hainan Univ, Coll Ocean, Haikou 570228, Peoples R China
KeywordAndrogen Receptor Sex Reversal Epinephelus Coioides
Subject AreaBiochemistry & Molecular Biology ; Zoology
Language英语
Funding ProjectLMB
WOS IDWOS:000307030000004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9838
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorlsslhr@mail.sysu.edu.cn
Recommended Citation
GB/T 7714
Shi, Y,Liu, XC,Zhang, HF,et al. Molecular identification of an androgen receptor and its changes in mRNA levels during 17 alpha-methyltestosterone-induced sex reversal in the orange-spotted grouper Epinephelus coioides[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2012,163(1):43-50.
APA Shi, Y.,Liu, XC.,Zhang, HF.,Zhang, Y.,Lu, DQ.,...&lsslhr@mail.sysu.edu.cn.(2012).Molecular identification of an androgen receptor and its changes in mRNA levels during 17 alpha-methyltestosterone-induced sex reversal in the orange-spotted grouper Epinephelus coioides.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,163(1),43-50.
MLA Shi, Y,et al."Molecular identification of an androgen receptor and its changes in mRNA levels during 17 alpha-methyltestosterone-induced sex reversal in the orange-spotted grouper Epinephelus coioides".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 163.1(2012):43-50.
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