中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Ecology; Environmental Sciences; Toxicology
题名: Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae)
作者: Shen, PP ; Zhou, H ; Gu, JG
通讯作者: pshen@scsio.ac.cn
关键词: Neanthes glandicincta ; Internal transcribed spacers ; Variation ; Polymorphism ; Phylogenetic analysis
刊名: ECOTOXICOLOGY
发表日期: 2012
卷: 21, 期:6, 页:1717-1725
部门归属: [Shen, Ping-Ping] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China; [Zhou, Hong] Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China; [Gu, Ji-Guang] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China
项目归属: LMB
摘要: Sequences of internal transcribed spacers (ITS1 and ITS2) are increasingly being used to infer phylogenetic relationships at or below species levels. Here we report a novel case of ITS polymorphism within Neanthes glandicincta (Annelida: Polychaeta: Nereididae). Two types of ITS sequence (Type I and Type II) were cloned and sequenced, which showed significant differences both in nucleotide composition and length. Variations of these two types sequences also differed from each other with Type I was highly divergent while Type II was highly conserved. Phylogenetic trees inferred from ITS1 and ITS2 sequences showed striking discrepancy in N. glandicincta. Non-concerted evolution of multi-gene is suggested to be responsible for the high degree of polymorphism in ITS regions. Due to the two divergent types of ITS presented within a single N. glandicincta individual, the utilization of ITS regions for delineation of population or closely related species cannot be substantiated. The finding of different types of ITS in a single individual also stresses the need for analyzing a large number of clones whenever ITS sequences obtained by PCR amplification and cloning are being used in phylogenetic reconstruction.
语种: 英语
WOS记录号: WOS:000306592100016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9850
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
s10646-012-0959-3.pdf(274KB)----开放获取--View Download

Recommended Citation:
Shen, PP; Zhou, H; Gu, JG.Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae),ECOTOXICOLOGY,2012,21(6):1717-1725
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Shen, PP]'s Articles
[Zhou, H]'s Articles
[Gu, JG]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Shen, PP]‘s Articles
[Zhou, H]‘s Articles
[Gu, JG]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: s10646-012-0959-3.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace