Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase
Zhu, YG; Fu, P; Lin, QH; Zhang, GT; Zhang, HB; Li, SM; Ju, JH; Zhu, WM; Zhang, CS; weimingzhu@ouc.edu.cn; czhang2006@gmail.com
2012
发表期刊ORGANIC LETTERS
ISSN1523-7060
卷号14期号:11页码:2666-2669
摘要The biosynthetic gene cluster for caerulomycin A (1) was cloned and characterized from the marine actinomycete Actinoalloteichus cyanogriseus WH1-2216-6, which revealed an unusual hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) system. The crmL disruption mutant accumulated caerulomycin L (2) with an extended L-leucine at C-7, implicating an amidohydrolase activity for CrmL. The leucine-removing activity was confirmed for crude CrmL enzymes. Heterologous expression of the 1 gene cluster led to 1 production in Streptomyces coelicolor.
部门归属[Fu, Peng; Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Minist Educ China, Qingdao 266003, Peoples R China; [Zhu, Yiguang; Lin, Qinheng; Zhang, Guangtao; Zhang, Haibo; Li, Sumei; Ju, Jianhua; Zhang, Changsheng] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med,RNAM Ctr Marine, CAS Key Lab Marine Bioresources Sustainable Utili, Guangzhou 510301, Guangdong, Peoples R China
学科领域Chemistry, Organic
语种英语
资助项目LMB
WOS记录号WOS:000304682700004
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9888
专题中科院海洋生物资源可持续利用重点实验室
通讯作者weimingzhu@ouc.edu.cn; czhang2006@gmail.com
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Zhu, YG,Fu, P,Lin, QH,et al. Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase[J]. ORGANIC LETTERS,2012,14(11):2666-2669.
APA Zhu, YG.,Fu, P.,Lin, QH.,Zhang, GT.,Zhang, HB.,...&czhang2006@gmail.com.(2012).Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase.ORGANIC LETTERS,14(11),2666-2669.
MLA Zhu, YG,et al."Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase".ORGANIC LETTERS 14.11(2012):2666-2669.
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