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学科主题: Biodiversity Conservation; Genetics & Heredity
题名: Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus
作者: Yuan, LH ; Xia, JJ ; Zhang, LP ; Hu, CQ
通讯作者: cqhu@scsio.ac.cn
关键词: Genetic structure ; Microsatellite loci ; Stichopus horrens
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2012
卷: 4, 期:2, 页:303-305
部门归属: [Yuan, Lihong; Xia, Jianjun; Zhang, Lvping; Hu, Chaoqun] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Lihong; Xia, Jianjun; Zhang, Lvping; Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Lvping] S China Inst Endangered Anim, Guangzhou 510260, Guangdong, Peoples R China
项目归属: LMB
摘要: This study employed the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the tropical sea cucumber species Stichopus horrens. Sixteen novel microsatellite loci were identified and we assessed the polymorphism of 40 individual samples from Hainan Province, China. The numbers of alleles ranged from 8 to 18 (total 213, mean 13.3), the observed and expected heterozygosity varied from 0.294 to 0.971, and from 0.720 to 0.913, and 12 of the 16 loci were found to be in accordance with the Hardy-Weinberg equilibrium. In addition, the successful cross-amplification of 8 loci with the congeneric Stichopus monotuberculatus, suggests that these markers could also be used in this species. These polymorphic markers can be useful for additional studies of population structure and stock management of S. horrens and related species.
语种: 英语
WOS记录号: WOS:000303536400022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9894
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Yuan, LH; Xia, JJ; Zhang, LP; Hu, CQ.Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus,CONSERVATION GENETICS RESOURCES,2012,4(2):303-305
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