Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus
Yuan, LH; Xia, JJ; Zhang, LP; Hu, CQ; cqhu@scsio.ac.cn
2012
发表期刊CONSERVATION GENETICS RESOURCES
ISSN1877-7252
卷号4期号:2页码:303-305
摘要This study employed the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the tropical sea cucumber species Stichopus horrens. Sixteen novel microsatellite loci were identified and we assessed the polymorphism of 40 individual samples from Hainan Province, China. The numbers of alleles ranged from 8 to 18 (total 213, mean 13.3), the observed and expected heterozygosity varied from 0.294 to 0.971, and from 0.720 to 0.913, and 12 of the 16 loci were found to be in accordance with the Hardy-Weinberg equilibrium. In addition, the successful cross-amplification of 8 loci with the congeneric Stichopus monotuberculatus, suggests that these markers could also be used in this species. These polymorphic markers can be useful for additional studies of population structure and stock management of S. horrens and related species.
部门归属[Yuan, Lihong; Xia, Jianjun; Zhang, Lvping; Hu, Chaoqun] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Lihong; Xia, Jianjun; Zhang, Lvping; Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov LAMB, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Lvping] S China Inst Endangered Anim, Guangzhou 510260, Guangdong, Peoples R China
关键词Genetic Structure Microsatellite Loci Stichopus Horrens
学科领域Biodiversity Conservation ; Genetics & Heredity
语种英语
资助项目LMB
WOS记录号WOS:000303536400022
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9894
专题中科院海洋生物资源可持续利用重点实验室
通讯作者cqhu@scsio.ac.cn
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Yuan, LH,Xia, JJ,Zhang, LP,et al. Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus[J]. CONSERVATION GENETICS RESOURCES,2012,4(2):303-305.
APA Yuan, LH,Xia, JJ,Zhang, LP,Hu, CQ,&cqhu@scsio.ac.cn.(2012).Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus.CONSERVATION GENETICS RESOURCES,4(2),303-305.
MLA Yuan, LH,et al."Development of 16 microsatellite loci of the tropical sea cucumber Stichopus horrens with cross-amplification in the congeneric species Stichopus monotuberculatus".CONSERVATION GENETICS RESOURCES 4.2(2012):303-305.
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