中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Chemistry, Multidisciplinary
题名: Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis
作者: Li, HX ; Zhang, QB ; Li, SM ; Zhu, YG ; Zhang, GT ; Zhang, HB ; Tian, XP ; Zhang, S ; Ju, JH ; Zhang, CS
通讯作者: czhang2006@gmail.com
刊名: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
发表日期: 2012
卷: 134, 期:21, 页:8996-9005
部门归属: [Li, Huixian; Zhang, Qingbo; Li, Sumei; Zhu, Yiguang; Zhang, Guangtao; Zhang, Haibo; Tian, Xinpeng; Zhang, Si; Ju, Jianhua; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Qingbo] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
项目归属: LMB
摘要: Xiamycin A (XMA) and oxiamycin (OXM) are bacterial indolosesquiterpenes featuring rare pentacyclic ring systems and are isolated from a marine-derived Streptomyces sp. SCSIO 02999. The putative biosynthetic gene cluster for XMA/OXM was identified by a partial genome sequencing approach. Eighteen genes were proposed to be involved in XMA/OXM biosynthesis, including five genes for terpene synthesis via a non-mevalonate pathway, eight genes encoding oxidoreductases, and five genes for regulation and resistance. Targeted disruptions of 13 genes within the xia gene cluster were carried out to probe their encoded functions in XMA/OXM biosynthesis. The disruption of xiaK, encoding an aromatic ring hydroxylase, led to a mutant producing indosespene and a minor amount of XMA. Feeding of indosespene to XMA/OXM nonproducing mutants revealed indosespene as a common precursor for XMA/OXM biosynthesis. Most notably, the flavin dependent oxygenase XiaI was biochemically characterized in vitro to convert indosespene to XMA, revealing an unusual oxidative cyclization strategy tailoring indolosesquiterpene biosynthesis.
语种: 英语
WOS记录号: WOS:000304570700044
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9898
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis.pdf(1727KB)----开放获取--View Download

Recommended Citation:
Li, HX; Zhang, QB; Li, SM; Zhu, YG; Zhang, GT; Zhang, HB; Tian, XP; Zhang, S; Ju, JH; Zhang, CS.Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis,JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(21):8996-9005
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, HX]'s Articles
[Zhang, QB]'s Articles
[Li, SM]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, HX]‘s Articles
[Zhang, QB]‘s Articles
[Li, SM]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace