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Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis
Li, HX; Zhang, QB; Li, SM; Zhu, YG; Zhang, GT; Zhang, HB; Tian, XP; Zhang, S; Ju, JH; Zhang, CS; czhang2006@gmail.com
2012
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume134Issue:21Pages:8996-9005
AbstractXiamycin A (XMA) and oxiamycin (OXM) are bacterial indolosesquiterpenes featuring rare pentacyclic ring systems and are isolated from a marine-derived Streptomyces sp. SCSIO 02999. The putative biosynthetic gene cluster for XMA/OXM was identified by a partial genome sequencing approach. Eighteen genes were proposed to be involved in XMA/OXM biosynthesis, including five genes for terpene synthesis via a non-mevalonate pathway, eight genes encoding oxidoreductases, and five genes for regulation and resistance. Targeted disruptions of 13 genes within the xia gene cluster were carried out to probe their encoded functions in XMA/OXM biosynthesis. The disruption of xiaK, encoding an aromatic ring hydroxylase, led to a mutant producing indosespene and a minor amount of XMA. Feeding of indosespene to XMA/OXM nonproducing mutants revealed indosespene as a common precursor for XMA/OXM biosynthesis. Most notably, the flavin dependent oxygenase XiaI was biochemically characterized in vitro to convert indosespene to XMA, revealing an unusual oxidative cyclization strategy tailoring indolosesquiterpene biosynthesis.
Department[Li, Huixian; Zhang, Qingbo; Li, Sumei; Zhu, Yiguang; Zhang, Guangtao; Zhang, Haibo; Tian, Xinpeng; Zhang, Si; Ju, Jianhua; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Qingbo] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject AreaChemistry, Multidisciplinary
Language英语
Funding ProjectLMB
WOS IDWOS:000304570700044
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9898
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorczhang2006@gmail.com
Recommended Citation
GB/T 7714
Li, HX,Zhang, QB,Li, SM,et al. Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(21):8996-9005.
APA Li, HX.,Zhang, QB.,Li, SM.,Zhu, YG.,Zhang, GT.,...&czhang2006@gmail.com.(2012).Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,134(21),8996-9005.
MLA Li, HX,et al."Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134.21(2012):8996-9005.
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