Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis
Li, HX; Zhang, QB; Li, SM; Zhu, YG; Zhang, GT; Zhang, HB; Tian, XP; Zhang, S; Ju, JH; Zhang, CS; czhang2006@gmail.com
2012
发表期刊JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
卷号134期号:21页码:8996-9005
摘要Xiamycin A (XMA) and oxiamycin (OXM) are bacterial indolosesquiterpenes featuring rare pentacyclic ring systems and are isolated from a marine-derived Streptomyces sp. SCSIO 02999. The putative biosynthetic gene cluster for XMA/OXM was identified by a partial genome sequencing approach. Eighteen genes were proposed to be involved in XMA/OXM biosynthesis, including five genes for terpene synthesis via a non-mevalonate pathway, eight genes encoding oxidoreductases, and five genes for regulation and resistance. Targeted disruptions of 13 genes within the xia gene cluster were carried out to probe their encoded functions in XMA/OXM biosynthesis. The disruption of xiaK, encoding an aromatic ring hydroxylase, led to a mutant producing indosespene and a minor amount of XMA. Feeding of indosespene to XMA/OXM nonproducing mutants revealed indosespene as a common precursor for XMA/OXM biosynthesis. Most notably, the flavin dependent oxygenase XiaI was biochemically characterized in vitro to convert indosespene to XMA, revealing an unusual oxidative cyclization strategy tailoring indolosesquiterpene biosynthesis.
部门归属[Li, Huixian; Zhang, Qingbo; Li, Sumei; Zhu, Yiguang; Zhang, Guangtao; Zhang, Haibo; Tian, Xinpeng; Zhang, Si; Ju, Jianhua; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Qingbo] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
学科领域Chemistry, Multidisciplinary
语种英语
资助项目LMB
WOS记录号WOS:000304570700044
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9898
专题中科院海洋生物资源可持续利用重点实验室
通讯作者czhang2006@gmail.com
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GB/T 7714
Li, HX,Zhang, QB,Li, SM,et al. Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(21):8996-9005.
APA Li, HX.,Zhang, QB.,Li, SM.,Zhu, YG.,Zhang, GT.,...&czhang2006@gmail.com.(2012).Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,134(21),8996-9005.
MLA Li, HX,et al."Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134.21(2012):8996-9005.
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