Novel Basic Protein, PfN23, Functions as Key Macromolecule during Nacre Formation
Fang, D; Pan, C; Lin, HJ; Lin, Y; Zhang, GY; Wang, HZ; He, MX; Xie, LP; Zhang, RQ; lpxie@mail.tsinghua.edu.cn; rqzhang@mail.tsinghua.edu.cn
2012
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
卷号287期号:19页码:15776-15785
摘要The fine microstructure of nacre (mother of pearl) illustrates the beauty of nature. Proteins found in nacre were believed to be "natural hands" that control nacre formation. In the classical view of nacre formation, nucleation of the main minerals, calcium carbonate, is induced on and by the acidic proteins in nacre. However, the basic proteins were not expected to be components of nacre. Here, we reported that a novel basic protein, PfN23, was a key accelerator in the control over crystal growth in nacre. The expression profile, in situ immunostaining, and in vitro immunodetection assays showed that PfN23 was localized within calcium carbonate crystals in the nacre. Knocking down the expression of PfN23 in adults via double-stranded RNA injection led to a disordered nacre surface in adults. Blocking the translation of PfN23 in embryos using morpholino oligomers led to the arrest of larval development. The in vitro crystallization assay showed that PfN23 increases the rate of calcium carbonate deposition and induced the formation of aragonite crystals with characteristics close to nacre. In addition, we constructed the peptides and truncations of different regions of this protein and found that the positively charged C-terminal region was a key region for the function of PfN23 Taken together, the basic protein PfN23 may be a key accelerator in the control of crystal growth in nacre. This provides a valuable balance to the classic view that acidic proteins control calcium carbonate deposition in nacre.
部门归属[Fang, Dong; Pan, Cong; Lin, Huijuan; Lin, Ya; Zhang, Guiyou; Wang, Hongzhong; Xie, Liping; Zhang, Rongqing] Tsinghua Univ, Sch Life Sci, Inst Marine Biotechnol, Beijing 100084, Peoples R China; [Fang, Dong; Pan, Cong; Lin, Huijuan; Lin, Ya; Zhang, Guiyou; Wang, Hongzhong; Xie, Liping; Zhang, Rongqing] Tsinghua Univ, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China; [He, Maoxian] Chinese Acad Sci, Guangzhou 510301, Guangdong, Peoples R China
学科领域Biochemistry & Molecular Biology
语种英语
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9902
专题中科院海洋生物资源可持续利用重点实验室
通讯作者lpxie@mail.tsinghua.edu.cn; rqzhang@mail.tsinghua.edu.cn
推荐引用方式
GB/T 7714
Fang, D,Pan, C,Lin, HJ,et al. Novel Basic Protein, PfN23, Functions as Key Macromolecule during Nacre Formation[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(19):15776-15785.
APA Fang, D.,Pan, C.,Lin, HJ.,Lin, Y.,Zhang, GY.,...&rqzhang@mail.tsinghua.edu.cn.(2012).Novel Basic Protein, PfN23, Functions as Key Macromolecule during Nacre Formation.JOURNAL OF BIOLOGICAL CHEMISTRY,287(19),15776-15785.
MLA Fang, D,et al."Novel Basic Protein, PfN23, Functions as Key Macromolecule during Nacre Formation".JOURNAL OF BIOLOGICAL CHEMISTRY 287.19(2012):15776-15785.
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