Characterization of Extended-Spectrum beta-Lactamase Genes Found among Escherichia coli Isolates from Duck and Environmental Samples Obtained on a Duck Farm
Ma, JY; Liu, JH; Lv, LC; Zong, ZY; Sun, Y; Zheng, HQ; Chen, ZL; Zeng, ZL; jhliu@scau.edu.cn; zizeng@scau.edu.cn
2012
发表期刊APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
卷号78期号:10页码:3668-3673
摘要In this study, we focused on evaluating the occurrence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in fecal samples of healthy ducks and environmental samples from a duck farm in South China. Duck cloacal swabs and pond water samples were cultivated on MacConkey agar plates supplemented with ceftiofur. Individual colonies were examined for ESBL production. Bacteria identified as E. coli were screened for the presence of FSBL and plasmid-borne AmpC genes. The genetic relatedness, plasmid replicon type, and genetic background were determined. Of 245 samples analyzed, 123 had E. coli isolates with ceftiofur MICs higher than 8 mu g/ml (116 [50.4%] from 230 duck samples and 7 [46.7%] from 15 water samples). bla(CTX-M), bla(SHV-12), bla(CMY-2), and bla(DHA-1) were identified in 108, 5, 9, and 1 isolates, respectively. The most common bla(CTX-M) genes were bla(CTX-M-27) (n = 34), bla(CTX-M-55) (n = 27), bla(CTX-M-24e) (n = 22), and bla(CTX-M-105) (n = 20), followed by bla(CTX-M-14a), bla(CTX-M-14b), bla(CTX-M-24a), and bla(CTX-M-24b). Although most of the CTX-M producers had distinct pulsotypes, clonal transmission between duck and water isolates was observed. bla(CTX-M) genes were carried by transferable IncN, IncF, and untypeable plasmids. The novel CTX-M gene bla(CTX-M-105), was flanked by two hypothetical protein sequences, partial ISEcp1 upstream and truncated IS903D, iroN, orf1, and a Tn1721-like element downstream. It is suggested that the horizontal transfer of bla(CTX-M) genes mediated by mobile elements and the clonal spread of CTX-M-producing E. coli isolates contributed to the dissemination of bla(CTX-M) in the duck farm. Our findings highlight the importance of ducks for the dissemination of transferable antibiotic resistance genes into the environment.
部门归属[Ma, Junying; Liu, Jian-Hua; Lv, Luchao; Sun, Yan; Zheng, Hongqing; Chen, ZhangLiu; Zeng, Zhen-Ling] S China Agr Univ, Coll Vet Med, Natl Reference Lab Vet Drug Residues SCAU, Guangzhou, Guangdong, Peoples R China; [Ma, Junying] Chinese Acad Sci, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, CAS Key Lab Marine Bioresources Sustainable Utili, Guangzhou, Guangdong, Peoples R China; [Zong, Zhiyong] Sichuan Univ, W China Hosp, Ctr Infect Dis, Chengdu 610064, Peoples R China
学科领域Biotechnology & Applied Microbiology ; Microbiology
语种英语
资助项目LMB
WOS记录号WOS:000303553900018
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9922
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jhliu@scau.edu.cn; zizeng@scau.edu.cn
推荐引用方式
GB/T 7714
Ma, JY,Liu, JH,Lv, LC,et al. Characterization of Extended-Spectrum beta-Lactamase Genes Found among Escherichia coli Isolates from Duck and Environmental Samples Obtained on a Duck Farm[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2012,78(10):3668-3673.
APA Ma, JY.,Liu, JH.,Lv, LC.,Zong, ZY.,Sun, Y.,...&zizeng@scau.edu.cn.(2012).Characterization of Extended-Spectrum beta-Lactamase Genes Found among Escherichia coli Isolates from Duck and Environmental Samples Obtained on a Duck Farm.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,78(10),3668-3673.
MLA Ma, JY,et al."Characterization of Extended-Spectrum beta-Lactamase Genes Found among Escherichia coli Isolates from Duck and Environmental Samples Obtained on a Duck Farm".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 78.10(2012):3668-3673.
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