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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)
作者: Guo, ML ; Wei, JG ; Huang, XH ; Huang, YH ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: beta-Defensin ; Antiviral effects ; Epinephelus coioides ; SGIV ; VNNV
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2012
卷: 32, 期:5, 页:828-838
部门归属: [Guo, Minglan; Wei, Jingguang; Huang, Xiaohong; Huang, Youhua; Qin, Qiwei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Defensins are a group of small antimicrobial peptides playing an important role in innate host defense. In this study, a beta-defensin cloned from liver of orange-spotted grouper, Epinephelus coioides, EcDefensin, showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (VNNV). The expression profiles of EcDefensin were significantly (P < 0.001) up-regulated after challenging with Lipopolysaccharide (LPS), SGIV and Polyriboinosinic Polyribocytidylic Acid (polyl:C) in vivo. Immunofluorescence staining observed its intracellular innate immune response to viral infection of SGIV and VNNV. EcDefensin was found to possess dual antiviral activity, inhibiting the infection and replication of SGIV and VNNV and inducting a type I interferon-related response in vitro. Synthetic peptide of EcDefensin (Ec-defensin) incubated with virus or cells before infection reduced the viral infectivity. Ec-defensin drastically decreased SGIV and VNNV titers, viral gene expression and structural protein accumulation. Grouper spleen cells over-expressing EcDefensin (GS/pcDNA-EcDefensin) support the inhibition of viral infection and the upregulation of the expression of host immune-related genes, such as antiviral protein Mx and pro-inflammatory cytokine IL-1 beta. EcDefensin activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. Reporter genes of IFN-Luc and ISRE-Luc were significantly up-regulated in cells transfected with pcDNA-EcDefenisn after infection with SGIV and VNNV. These results suggest that EcDefensin is importantly involved in host immune responses to invasion of viral pathogens, and open the new avenues for design of antiviral agents in fisheries industry. (C) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
WOS记录号: WOS:000302667200025
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9934
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Guo, ML; Wei, JG; Huang, XH; Huang, YH; Qin, QW.Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides),FISH & SHELLFISH IMMUNOLOGY,2012,32(5):828-838
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