Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)
Guo, ML; Wei, JG; Huang, XH; Huang, YH; Qin, QW; qinqw@scsio.ac.cn
2012
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号32期号:5页码:828-838
摘要Defensins are a group of small antimicrobial peptides playing an important role in innate host defense. In this study, a beta-defensin cloned from liver of orange-spotted grouper, Epinephelus coioides, EcDefensin, showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (VNNV). The expression profiles of EcDefensin were significantly (P < 0.001) up-regulated after challenging with Lipopolysaccharide (LPS), SGIV and Polyriboinosinic Polyribocytidylic Acid (polyl:C) in vivo. Immunofluorescence staining observed its intracellular innate immune response to viral infection of SGIV and VNNV. EcDefensin was found to possess dual antiviral activity, inhibiting the infection and replication of SGIV and VNNV and inducting a type I interferon-related response in vitro. Synthetic peptide of EcDefensin (Ec-defensin) incubated with virus or cells before infection reduced the viral infectivity. Ec-defensin drastically decreased SGIV and VNNV titers, viral gene expression and structural protein accumulation. Grouper spleen cells over-expressing EcDefensin (GS/pcDNA-EcDefensin) support the inhibition of viral infection and the upregulation of the expression of host immune-related genes, such as antiviral protein Mx and pro-inflammatory cytokine IL-1 beta. EcDefensin activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. Reporter genes of IFN-Luc and ISRE-Luc were significantly up-regulated in cells transfected with pcDNA-EcDefenisn after infection with SGIV and VNNV. These results suggest that EcDefensin is importantly involved in host immune responses to invasion of viral pathogens, and open the new avenues for design of antiviral agents in fisheries industry. (C) 2012 Elsevier Ltd. All rights reserved.
部门归属[Guo, Minglan; Wei, Jingguang; Huang, Xiaohong; Huang, Youhua; Qin, Qiwei] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
关键词Beta-defensin Antiviral Effects Epinephelus Coioides Sgiv Vnnv
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
资助项目LMB
WOS记录号WOS:000302667200025
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9934
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Guo, ML,Wei, JG,Huang, XH,et al. Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(5):828-838.
APA Guo, ML,Wei, JG,Huang, XH,Huang, YH,Qin, QW,&qinqw@scsio.ac.cn.(2012).Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,32(5),828-838.
MLA Guo, ML,et al."Antiviral effects of beta-defensin derived from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 32.5(2012):828-838.
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