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A comparative study of bacterial community structures in the sediments from brominated flame retardants contaminated river and non-contaminated reservoir
Wu, HB; Zhao, HZ; Wen, C; Guo, YT; Guo, J; Xu, MY; Li, X; wgh@scsio.ac.cn
2012
Source PublicationAFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
ISSN1996-0808
Volume6Issue:13Pages:3248-3260
AbstractThe bacterial community structure in sediments was studied by rapid amplified ribosomal DNA restriction analysis (ARDRA) combined with phylogenetic analysis of 16S rDNA clone libraries. Comparative analyses were carried out on two sediment samples collected from a non-contaminated reservoir, and a brominated flame retardants contaminated Lian River, and two sediment samples enriched with decabromodiphenyl oxide for one and two months. Based on the bacterial diversity, sediments from the reservoir and Lian River contained a more diversified bacterial population compared to those after enrichment. The composition of bacterial strains, the dominant strains, and the number of gene clones identified to each family of bacteria which significantly differed between sediments from the contaminated river and non-contaminated reservoir. From each of the two types of sediments, 100 rDNA clones had matched sequences in the GenBank databases, 91 clones were in the unculturable bacteria category, and 43 clones possessed less than 95% sequence similarity. In the two enriched samples, the bacterial population structure became less diversified. 28 out of 82 sequences matched with sequences of unculturable origin with 17 clones sharing less than 95% homology with known sequence deposited at the GenBank. Compared to the original river sediments, the bacterial community composition was altered after the enrichment. The enriched samples contained fewer phyla, but more abundant within-phyla members of bacteria. The dominant strains are in the classes of a-and beta proteobacteria, and the ratio of culturable bacteria was also increased. Results from this study indicate that different contaminants can cause alteration in the bacterial community structure.
Department[Wu, Houbo; Zhao, Hengzhi; Wen, Chen; Guo, Yatao; Li, Xiang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, S China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med LMMM GD, Guangzhou 510301, Guangdong, Peoples R China; [Zhao, Hengzhi; Wen, Chen; Guo, Yatao] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Guo, Jun; Xu, Meiying] Guangdong Inst Microbiol, Guangzhou 510070, Guangdong, Peoples R China
KeywordBrominated Flame Retardants Decabromodiphenyl Oxide Bacterial Diversity Amplified Ribosomal Dna Restriction Analysis (Ardra)
Subject AreaMicrobiology
Language英语
Funding ProjectLMB
WOS IDWOS:000303813500023
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9938
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorwgh@scsio.ac.cn
Recommended Citation
GB/T 7714
Wu, HB,Zhao, HZ,Wen, C,et al. A comparative study of bacterial community structures in the sediments from brominated flame retardants contaminated river and non-contaminated reservoir[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2012,6(13):3248-3260.
APA Wu, HB.,Zhao, HZ.,Wen, C.,Guo, YT.,Guo, J.,...&wgh@scsio.ac.cn.(2012).A comparative study of bacterial community structures in the sediments from brominated flame retardants contaminated river and non-contaminated reservoir.AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,6(13),3248-3260.
MLA Wu, HB,et al."A comparative study of bacterial community structures in the sediments from brominated flame retardants contaminated river and non-contaminated reservoir".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 6.13(2012):3248-3260.
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