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学科主题: Fisheries; Marine & Freshwater Biology
题名: IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE
作者: Yuan, T ; He, MX ; Huang, LM
通讯作者: hmx@scsio.ac.cn
关键词: scallop ; Chlamys nobilis Reeve ; AFLP ; shell color ; genetic control
刊名: JOURNAL OF SHELLFISH RESEARCH
发表日期: 2012
卷: 31, 期:1, 页:33-37
部门归属: [Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, LAMB, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: The DNA amplified fragment length polymorphism (AFLP) fingerprinting approach was used to identify a shell color-linked marker in the noble scallop Chlamys nobilis Reeve. The marker BIfI76 was linked completely to the gene for brigh-torange shell color with no recombination in the progeny we sampled. This marker was amplified in bright-orange parents and all orange progeny, but was absent in all of the brownish purple progeny. Nevertheless, A FLP markers are not feasible for large-scale application. Thus, we converted BIfI76 into a sequence-characterized amplified region (SCAR) marker that we designated as SCAR Orange-142. The marker Orange-142, like the A FLP band from which it was derived, amplified a robust band in only the bright-orange scallops, both in F, and F-2 families as well as in a test group obtained from a seafood market. These results indicate that Orange-142 is tightly linked to the gene for orange shell color. In all but 1 of 10 F2 families derived from bright-orange F-o grandparents, the observed frequencies of orange progeny were consistent with Mendelian ratios expected from a I-locus 2-allele system in which the brownish purple genotype was recessive. This study is the first report of the genetic basis for shell color in C. nobilis. The identification of a shell color-linked marker in the noble scallop improves our understanding of shell color inheritance and may contribute to C. nobilis broodstock enhancement programs using marker-assisted selection.
语种: 英语
WOS记录号: WOS:000302846800004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9948
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Yuan, T; He, MX; Huang, LM.IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE,JOURNAL OF SHELLFISH RESEARCH,2012,31(1):33-37
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文件名: IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE.pdf
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