IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE
Yuan, T; He, MX; Huang, LM; hmx@scsio.ac.cn
2012
发表期刊JOURNAL OF SHELLFISH RESEARCH
ISSN0730-8000
卷号31期号:1页码:33-37
摘要The DNA amplified fragment length polymorphism (AFLP) fingerprinting approach was used to identify a shell color-linked marker in the noble scallop Chlamys nobilis Reeve. The marker BIfI76 was linked completely to the gene for brigh-torange shell color with no recombination in the progeny we sampled. This marker was amplified in bright-orange parents and all orange progeny, but was absent in all of the brownish purple progeny. Nevertheless, A FLP markers are not feasible for large-scale application. Thus, we converted BIfI76 into a sequence-characterized amplified region (SCAR) marker that we designated as SCAR Orange-142. The marker Orange-142, like the A FLP band from which it was derived, amplified a robust band in only the bright-orange scallops, both in F, and F-2 families as well as in a test group obtained from a seafood market. These results indicate that Orange-142 is tightly linked to the gene for orange shell color. In all but 1 of 10 F2 families derived from bright-orange F-o grandparents, the observed frequencies of orange progeny were consistent with Mendelian ratios expected from a I-locus 2-allele system in which the brownish purple genotype was recessive. This study is the first report of the genetic basis for shell color in C. nobilis. The identification of a shell color-linked marker in the noble scallop improves our understanding of shell color inheritance and may contribute to C. nobilis broodstock enhancement programs using marker-assisted selection.
部门归属[Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China; [Yuan, Tao; He, Maoxian; Huang, Liangmin] Chinese Acad Sci, S China Sea Inst Oceanol, LAMB, Guangzhou 510301, Guangdong, Peoples R China
关键词Scallop Chlamys Nobilis Reeve Aflp Shell Color Genetic Control
学科领域Fisheries ; Marine & Freshwater Biology
语种英语
资助项目LMB
WOS记录号WOS:000302846800004
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9948
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn
推荐引用方式
GB/T 7714
Yuan, T,He, MX,Huang, LM,et al. IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE[J]. JOURNAL OF SHELLFISH RESEARCH,2012,31(1):33-37.
APA Yuan, T,He, MX,Huang, LM,&hmx@scsio.ac.cn.(2012).IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE.JOURNAL OF SHELLFISH RESEARCH,31(1),33-37.
MLA Yuan, T,et al."IDENTIFICATION OF AN AFLP FRAGMENT LINKED TO SHELL COLOR IN THE NOBLE SCALLOP CHLAMYS NOBILIS REEVE".JOURNAL OF SHELLFISH RESEARCH 31.1(2012):33-37.
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