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学科主题: Biotechnology & Applied Microbiology; Microbiology
题名: Characterization of the Amicetin Biosynthesis Gene Cluster from Streptomyces vinaceusdrappus NRRL 2363 Implicates Two Alternative Strategies for Amide Bond Formation
作者: Zhang, GY ; Zhang, HB ; Li, SM ; Xiao, J ; Zhang, GT ; Zhu, YG ; Niu, SW ; Ju, JH ; Zhang, CS
通讯作者: czhang2006@gmail.com
刊名: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
发表日期: 2012
卷: 78, 期:7, 页:2393-2401
部门归属: [Zhang, Gaiyun; Zhang, Haibo; Li, Sumei; Xiao, Ji; Zhang, Guangtao; Zhu, Yiguang; Niu, Siwen; Ju, Jianhua; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou, Guangdong, Peoples R China
项目归属: LMB
摘要: Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an alpha-(1 -> 4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthesis gene cluster was cloned from Streptomyces vinaceusdrappus NRRL 2363 and localized on a 37-kb contiguous DNA region. Heterologous expression of the amicetin biosynthesis gene cluster in Streptomyces lividans TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of the amicetin skeleton by the linkage of cytosine, p-aminobenzoic acid (PABA), and the terminal (+)-alpha-methylserine moieties. The inactivation of the benzoate coenzyme A (benzoate-CoA) ligase gene amiL and the N-acetyltransferase gene amiF led to two mutants that accumulated the same two compounds, cytosamine and 4-acetamido-3-hydroxybenzoic acid. These data indicated that AmiF functioned as an amide synthethase to link cytosine and PABA. The inactivation of amiR, encoding an acyl-CoA-acyl carrier protein transacylase, resulted in the production of plicacetin and norplicacetin, indicating AmiR to be responsible for attachment of the terminal methylserine moiety to form another amide bond. These findings implicated two alternative strategies for amide bond formation in amicetin biosynthesis.
语种: 英语
WOS记录号: WOS:000301344300037
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9954
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Zhang, GY; Zhang, HB; Li, SM; Xiao, J; Zhang, GT; Zhu, YG; Niu, SW; Ju, JH; Zhang, CS.Characterization of the Amicetin Biosynthesis Gene Cluster from Streptomyces vinaceusdrappus NRRL 2363 Implicates Two Alternative Strategies for Amide Bond Formation,APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2012,78(7):2393-2401
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文件名: Characterization of the Amicetin Biosynthesis Gene Cluster from Streptomyces vinaceusdrappus NRRL 2363 Implicates Two Alternative Strategies for Amide Bond Formation.pdf
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