Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans
Ma, JY; Zuo, DG; Song, YX; Wang, B; Huang, HB; Yao, YL; Li, WJ; Zhang, S; Zhang, CS; Ju, JH; jju@scsio.ac.cn
2012
发表期刊CHEMBIOCHEM
ISSN1439-4227
卷号13期号:4页码:547-552
摘要The nine-membered indolactam antibiotics belong to a small group of antibiotics showing broad biological activities. However, the in vivo genetic engineering of compounds of this type has not been performed. Here we report the identification of a single gene cluster responsible for the biosynthesis of methylpendolmycin and pendolmycin, two members of this family of antibiotics, from the deep sea bacterium Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis and gene inactivation, coupled with metabolite characterization, reveal that MpnB, a nonribosomal peptide synthetase, MpnC, a cytochrome P450, and MpnD, a prenyltransferase, are sufficient to catalyze the biosynthesis of the two antibiotics from L-Ile (or L-Val), L-Trp, and methionine. MpnD is the first identified enzyme that transfers a C5 prenyl unit in a reverse manner to the C-7 position of a Trp-derived natural product.
部门归属[Ma, Junying; Zuo, Dianguang; Song, Yongxiang; Wang, Bo; Huang, Hongbo; Yao, Yueliang; Zhang, Si; Zhang, Changsheng; Ju, Jianhua] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Zuo, Dianguang] Chinese Acad Sci, Grad Univ, Beijing 110039, Peoples R China; [Li, Wenjun] Yunnan Univ, Yunnan Inst Microbiol, Kunming 650091, Peoples R China
关键词Biosynthesis Methylpendolmycin Natural Products Pendolmycin Prenyltransferases
学科领域Biochemistry & Molecular Biology ; Chemistry, Medicinal
语种英语
资助项目LMB
WOS记录号WOS:000300871600013
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9958
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jju@scsio.ac.cn
推荐引用方式
GB/T 7714
Ma, JY,Zuo, DG,Song, YX,et al. Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans[J]. CHEMBIOCHEM,2012,13(4):547-552.
APA Ma, JY.,Zuo, DG.,Song, YX.,Wang, B.,Huang, HB.,...&jju@scsio.ac.cn.(2012).Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans.CHEMBIOCHEM,13(4),547-552.
MLA Ma, JY,et al."Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans".CHEMBIOCHEM 13.4(2012):547-552.
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