中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biochemistry & Molecular Biology; Chemistry, Medicinal
题名: Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans
作者: Ma, JY ; Zuo, DG ; Song, YX ; Wang, B ; Huang, HB ; Yao, YL ; Li, WJ ; Zhang, S ; Zhang, CS ; Ju, JH
通讯作者: jju@scsio.ac.cn
关键词: biosynthesis ; methylpendolmycin ; natural products ; pendolmycin ; prenyltransferases
刊名: CHEMBIOCHEM
发表日期: 2012
卷: 13, 期:4, 页:547-552
部门归属: [Ma, Junying; Zuo, Dianguang; Song, Yongxiang; Wang, Bo; Huang, Hongbo; Yao, Yueliang; Zhang, Si; Zhang, Changsheng; Ju, Jianhua] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Zuo, Dianguang] Chinese Acad Sci, Grad Univ, Beijing 110039, Peoples R China; [Li, Wenjun] Yunnan Univ, Yunnan Inst Microbiol, Kunming 650091, Peoples R China
项目归属: LMB
摘要: The nine-membered indolactam antibiotics belong to a small group of antibiotics showing broad biological activities. However, the in vivo genetic engineering of compounds of this type has not been performed. Here we report the identification of a single gene cluster responsible for the biosynthesis of methylpendolmycin and pendolmycin, two members of this family of antibiotics, from the deep sea bacterium Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis and gene inactivation, coupled with metabolite characterization, reveal that MpnB, a nonribosomal peptide synthetase, MpnC, a cytochrome P450, and MpnD, a prenyltransferase, are sufficient to catalyze the biosynthesis of the two antibiotics from L-Ile (or L-Val), L-Trp, and methionine. MpnD is the first identified enzyme that transfers a C5 prenyl unit in a reverse manner to the C-7 position of a Trp-derived natural product.
语种: 英语
WOS记录号: WOS:000300871600013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9958
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Ma, JY; Zuo, DG; Song, YX; Wang, B; Huang, HB; Yao, YL; Li, WJ; Zhang, S; Zhang, CS; Ju, JH.Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans,CHEMBIOCHEM,2012,13(4):547-552
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma, JY]'s Articles
[Zuo, DG]'s Articles
[Song, YX]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma, JY]‘s Articles
[Zuo, DG]‘s Articles
[Song, YX]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace