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Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans
Ma, JY; Zuo, DG; Song, YX; Wang, B; Huang, HB; Yao, YL; Li, WJ; Zhang, S; Zhang, CS; Ju, JH; jju@scsio.ac.cn
2012
Source PublicationCHEMBIOCHEM
ISSN1439-4227
Volume13Issue:4Pages:547-552
AbstractThe nine-membered indolactam antibiotics belong to a small group of antibiotics showing broad biological activities. However, the in vivo genetic engineering of compounds of this type has not been performed. Here we report the identification of a single gene cluster responsible for the biosynthesis of methylpendolmycin and pendolmycin, two members of this family of antibiotics, from the deep sea bacterium Marinactinospora thermotolerans SCSIO 00652. Bioinformatics analysis and gene inactivation, coupled with metabolite characterization, reveal that MpnB, a nonribosomal peptide synthetase, MpnC, a cytochrome P450, and MpnD, a prenyltransferase, are sufficient to catalyze the biosynthesis of the two antibiotics from L-Ile (or L-Val), L-Trp, and methionine. MpnD is the first identified enzyme that transfers a C5 prenyl unit in a reverse manner to the C-7 position of a Trp-derived natural product.
Department[Ma, Junying; Zuo, Dianguang; Song, Yongxiang; Wang, Bo; Huang, Hongbo; Yao, Yueliang; Zhang, Si; Zhang, Changsheng; Ju, Jianhua] Chinese Acad Sci, CAS Key Lab Marine Bioresources Sustainable Utili, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,S China Sea Inst Oceano, Guangzhou 510301, Guangdong, Peoples R China; [Zuo, Dianguang] Chinese Acad Sci, Grad Univ, Beijing 110039, Peoples R China; [Li, Wenjun] Yunnan Univ, Yunnan Inst Microbiol, Kunming 650091, Peoples R China
KeywordBiosynthesis Methylpendolmycin Natural Products Pendolmycin Prenyltransferases
Subject AreaBiochemistry & Molecular Biology ; Chemistry, Medicinal
Language英语
Funding ProjectLMB
WOS IDWOS:000300871600013
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9958
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorjju@scsio.ac.cn
Recommended Citation
GB/T 7714
Ma, JY,Zuo, DG,Song, YX,et al. Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans[J]. CHEMBIOCHEM,2012,13(4):547-552.
APA Ma, JY.,Zuo, DG.,Song, YX.,Wang, B.,Huang, HB.,...&jju@scsio.ac.cn.(2012).Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans.CHEMBIOCHEM,13(4),547-552.
MLA Ma, JY,et al."Characterization of a Single Gene Cluster Responsible for Methylpendolmycin and Pendolmycin Biosynthesis in the Deep Sea Bacterium Marinactinospora thermotolerans".CHEMBIOCHEM 13.4(2012):547-552.
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