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学科主题: Biotechnology & Applied Microbiology; Microbiology
题名: Detection and characterization of two insertion sequences in Vibrio alginolyticus
作者: Ren, CH ; Jiang, X ; Sun, HY ; Luo, P ; Chen, C ; Zhao, Z ; Hu, CQ
通讯作者: cqhu@scsio.ac.cn
关键词: Insertion sequences ; Vibrio alginolyticus ; Detection ; Characterization
刊名: ANNALS OF MICROBIOLOGY
发表日期: 2012
卷: 62, 期:1, 页:69-75
部门归属: [Ren, Chunhua; Jiang, Xiao; Sun, Hongyan; Luo, Peng; Chen, Chang; Zhao, Zhe; Hu, Chaoqun] CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Ren, Chunhua; Jiang, Xiao; Sun, Hongyan; Luo, Peng; Chen, Chang; Zhao, Zhe; Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Ren, Chunhua; Jiang, Xiao; Sun, Hongyan; Luo, Peng; Chen, Chang; Zhao, Zhe; Hu, Chaoqun] Chinese Acad Sci, S China Sea Inst Oceanol, Chinese Acad Sci LAMB, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
摘要: Vibrio alginolyticus is the dominant Vibrio species and an opportunistic pathogen for marine animals and human. It has been reported to be an important reservoir for virulence genes in aquatic environment. In this study, a total of 187 isolates of V. alginolyticus from fish ponds or coral reefs in the South China Sea were examined for the presence of two insertion sequences, ISVal-1 and ISVal-2. In addition, the characteristics of the two ISs were described. The results showed that 58.82% of the examined isolates contained ISVal-1 and 23.53% had ISVal-2. For both ISVal-1 and ISVal-2, the detection rate for ISs from the fish ponds was lower than that from the coral reef. Sequence analysis revealed that ISVal-1 had the typical features of the IS5 family which contained 19 bp terminal inverted repeats (IRs) and a large open reading frame containing a conserved DDE motif. ISVal-2 possessed genetic structures identical to the IS3 family which had imperfect IRs and two partially overlapping reading frames, OrfA and orfB. A putative helix-turn-helix (HTH) motif, a presumed leucine zipper (LZ) motif, and a conserved DDE motif were contained in the two Orfs. In addition, the data suggested that ISVal-1 and ISVal-2 in V. alginolyticus may be horizontally transferred from other Vibrio species and play certain roles in the virulence of the pathogen. To our knowledge, this is the first report on ISs in V. alginolyticus.
语种: 英语
WOS记录号: WOS:000300605800010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/9968
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Ren, CH; Jiang, X; Sun, HY; Luo, P; Chen, C; Zhao, Z; Hu, CQ.Detection and characterization of two insertion sequences in Vibrio alginolyticus,ANNALS OF MICROBIOLOGY,2012,62(1):69-75
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