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Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.
Li, J; Zhao, GZ; Huang, HY; Qin, S; Zhu, WY; Zhao, LX; Xu, LH; Zhang, S; Li, WJ; Strobel, G; wjli@ynu.edu.cn
2012
Source PublicationANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
Volume101Issue:3Pages:515-527
AbstractEndophytic actinobacteria isolated from Artemisia annua were characterized and evaluated for their bioactivities. A total of 228 isolates representing at least 19 different genera of actinobacteria were obtained and several of them seemed to be novel taxa. An evaluation of antimicrobial activity showed that more isolates possessed activity towards plant pathogens than activity against other pathogenic bacteria or yeasts. High frequencies of PCR amplification were obtained for type I polyketide synthases (PKS-I, 21.1%), type II polyketide synthases (PKS-II, 45.2%) and nonribosomal peptide synthetases (NRPS, 32.5%). The results of herbicidal activity screening indicated that 19 out of 117 samples of fermentation broths completely inhibited the germination of Echinochloa crusgalli. This study indicated that endophytic actinobacteria associated with A. annua are abundant and have potentially beneficial and diverse bioactivities which should be pursued for their biotechnical promise.
Department[Li, Jie; Zhao, Guo-Zhen; Huang, Hai-Yu; Zhu, Wen-Yong; Zhao, Li-Xing; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Yunnan Inst Microbiol, Minist Educ, Key Lab Microbial Divers SW China, Kunming 650091, Peoples R China; [Li, Jie; Zhao, Guo-Zhen; Huang, Hai-Yu; Zhu, Wen-Yong; Zhao, Li-Xing; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Yunnan Inst Microbiol, Lab Conservat & Utilizat Bioresources, Kunming 650091, Peoples R China; [Li, Jie; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,S China Sea Inst, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Sheng] Xuzhou Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China; [Strobel, Gary] Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA
KeywordEndophytic Actinobacteria Artemisia Annua Diversity Antimicrobial Activity Herbicidal Activity Functional Genes
Subject AreaMicrobiology
Language英语
Funding ProjectLMB
WOS IDWOS:000300314200006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/9972
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorwjli@ynu.edu.cn
Recommended Citation
GB/T 7714
Li, J,Zhao, GZ,Huang, HY,et al. Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2012,101(3):515-527.
APA Li, J.,Zhao, GZ.,Huang, HY.,Qin, S.,Zhu, WY.,...&wjli@ynu.edu.cn.(2012).Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L..ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,101(3),515-527.
MLA Li, J,et al."Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 101.3(2012):515-527.
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