Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.
Li, J; Zhao, GZ; Huang, HY; Qin, S; Zhu, WY; Zhao, LX; Xu, LH; Zhang, S; Li, WJ; Strobel, G; wjli@ynu.edu.cn
2012
发表期刊ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
卷号101期号:3页码:515-527
摘要Endophytic actinobacteria isolated from Artemisia annua were characterized and evaluated for their bioactivities. A total of 228 isolates representing at least 19 different genera of actinobacteria were obtained and several of them seemed to be novel taxa. An evaluation of antimicrobial activity showed that more isolates possessed activity towards plant pathogens than activity against other pathogenic bacteria or yeasts. High frequencies of PCR amplification were obtained for type I polyketide synthases (PKS-I, 21.1%), type II polyketide synthases (PKS-II, 45.2%) and nonribosomal peptide synthetases (NRPS, 32.5%). The results of herbicidal activity screening indicated that 19 out of 117 samples of fermentation broths completely inhibited the germination of Echinochloa crusgalli. This study indicated that endophytic actinobacteria associated with A. annua are abundant and have potentially beneficial and diverse bioactivities which should be pursued for their biotechnical promise.
部门归属[Li, Jie; Zhao, Guo-Zhen; Huang, Hai-Yu; Zhu, Wen-Yong; Zhao, Li-Xing; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Yunnan Inst Microbiol, Minist Educ, Key Lab Microbial Divers SW China, Kunming 650091, Peoples R China; [Li, Jie; Zhao, Guo-Zhen; Huang, Hai-Yu; Zhu, Wen-Yong; Zhao, Li-Xing; Xu, Li-Hua; Li, Wen-Jun] Yunnan Univ, Yunnan Inst Microbiol, Lab Conservat & Utilizat Bioresources, Kunming 650091, Peoples R China; [Li, Jie; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,S China Sea Inst, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Sheng] Xuzhou Normal Univ, Key Lab Biotechnol Med Plant Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China; [Strobel, Gary] Montana State Univ, Dept Plant Sci, Bozeman, MT 59717 USA
关键词Endophytic Actinobacteria Artemisia Annua Diversity Antimicrobial Activity Herbicidal Activity Functional Genes
学科领域Microbiology
语种英语
资助项目LMB
WOS记录号WOS:000300314200006
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9972
专题中科院海洋生物资源可持续利用重点实验室
通讯作者wjli@ynu.edu.cn
推荐引用方式
GB/T 7714
Li, J,Zhao, GZ,Huang, HY,et al. Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2012,101(3):515-527.
APA Li, J.,Zhao, GZ.,Huang, HY.,Qin, S.,Zhu, WY.,...&wjli@ynu.edu.cn.(2012).Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L..ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,101(3),515-527.
MLA Li, J,et al."Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 101.3(2012):515-527.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
s10482-011-9661-3.pd(369KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。