Delta(11,12) Double Bond Formation in Tirandamycin Biosynthesis is Atypically Catalyzed by TrdE, a Glycoside Hydrolase Family Enzyme
Mo, XH; Ma, JY; Huang, HB; Wang, B; Song, YD; Zhang, S; Zhang, CS; Ju, JH; jju@scsio.ac.cn
2012
发表期刊JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
卷号134期号:6页码:2844-2847
摘要The tirandamycins (TAMs) are a small group of Streptomyces-derived natural products that target bacterial RNA polymerase. Within the TAM biosynthetic cluster, trdE encodes a glycoside hydrolase whose role in TAM biosynthesis has been undefined until now. We report that in vivo trdE inactivation leads to accumulation of pre-tirandamycin, the earliest intermediate released from its mixed polyketide/nonribosomal peptide biosynthetic assembly line. In vitro and site-directed mutagenesis studies showed that TrdE, a putative glycoside hydrolase, catalyzes in a highly atypical fashion the installation of the Delta(11,12) double bond during TAM biosynthesis.
部门归属[Mo, Xuhua; Ma, Junying; Huang, Hongbo; Wang, Bo; Song, Yongdang; Zhang, Si; Zhang, Changsheng; Ju, Jianhua] Chinese Acad Sci, S China Sea Inst Oceanol, RNAM Ctr Marine Microbiol,Guandong Key Lab Marine, CAS Key Lab Marine Bioresources Sustainable Utili, Guangzhou 510301, Guangdong, Peoples R China; [Mo, Xuhua] Chinese Acad Sci, Grad Univ, Beijing 110039, Peoples R China
学科领域Chemistry, Multidisciplinary
语种英语
资助项目LMB
WOS记录号WOS:000301161500001
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9974
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jju@scsio.ac.cn
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GB/T 7714
Mo, XH,Ma, JY,Huang, HB,et al. Delta(11,12) Double Bond Formation in Tirandamycin Biosynthesis is Atypically Catalyzed by TrdE, a Glycoside Hydrolase Family Enzyme[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2012,134(6):2844-2847.
APA Mo, XH.,Ma, JY.,Huang, HB.,Wang, B.,Song, YD.,...&jju@scsio.ac.cn.(2012).Delta(11,12) Double Bond Formation in Tirandamycin Biosynthesis is Atypically Catalyzed by TrdE, a Glycoside Hydrolase Family Enzyme.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,134(6),2844-2847.
MLA Mo, XH,et al."Delta(11,12) Double Bond Formation in Tirandamycin Biosynthesis is Atypically Catalyzed by TrdE, a Glycoside Hydrolase Family Enzyme".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 134.6(2012):2844-2847.
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