Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus
Chen, C; Wang, QB; Liu, ZH; Zhao, JJ; Jiang, X; Sun, HY; Ren, CH; Hu, CQ; cqhu@scsio.ac.cn
2012
发表期刊ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
卷号101期号:2页码:281-288
摘要toxR, a conserved virulence-associated gene in vibrios, is identified in Vibrio alginolyticus ZJ51-O, a pathogenic strain isolated from diseased fish. To reveal the role of ToxR in the pathogenicity of V. alginolyticus, a deletion mutant was constructed by allelic exchange. The mutant showed the same level of growth in trypticase soy broth (TSB) and iron-limiting condition, as the wild type strain. However, deletion of toxR severely reduced resistance against bile salts and the capability of biofilm formation. Outer-membrane protein (OMP) analysis showed that a 37-kD protein was absent and a 43-kD protein was decreased in the mutant. By MS/MS, the two proteins are identified as the homologues of OmpT and OmpN, respectively. These data suggest that ToxR might have enhanced the bile resistance and biofilm formation through modulating the production of OMP without affecting the ability of iron acquisition and the virulence to the fish via injection. These results indicate that ToxR may assist V. alginolyticus to colonize on the surface of the fish intestine which is crucial for the initiation of the infection, though it may not be involved in the proliferation of the bacteria in the host tissue.
部门归属[Chen Chang; Wang Qing-bai; Liu Zhu-Hong; Zhao Jing-jing; Jiang Xiao; Sun Hong-yan; Ren Chun-hua; Hu Chao-qun] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
关键词Vibrio Alginolyticus Toxr Bile Resistance Outer-membrane Protein Pathogenicity
学科领域Microbiology
语种英语
资助项目LMB
WOS记录号WOS:000299293900007
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/9984
专题中科院海洋生物资源可持续利用重点实验室
通讯作者cqhu@scsio.ac.cn
推荐引用方式
GB/T 7714
Chen, C,Wang, QB,Liu, ZH,et al. Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2012,101(2):281-288.
APA Chen, C.,Wang, QB.,Liu, ZH.,Zhao, JJ.,Jiang, X.,...&cqhu@scsio.ac.cn.(2012).Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,101(2),281-288.
MLA Chen, C,et al."Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 101.2(2012):281-288.
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